SINOPSIS

Productivity and nutritional contents in culinary-medicinal oyster mushroom Pleurotus ostreatus strain MBFBL67 cultivated on substrates containing anaerobic digester solid waste (SW) from poultry litter and wheat straw, with or without millet, were studied. Among the seven different combinations tested, a substrate combination consisting of 10% SW, 10% millet, and 80% wheat straw showed the best mushroom yield (1059.3 g Kg⁻¹, resulting in a biological efficiency [BE] of 105.9%). Fruit bodies collected were analyzed for total fat, protein, amino acids, and mineral elements. Mushrooms from substrate combinations (SCs) containing SW, wheat straw, and millet presented a protein content ranging between 29.6% and 37%. The total content of amino acids varied from 17.1 % (SC3) to 29.5% (SC7), among which essential amino acids corresponded to 31.8%−35.7% of the total content in all SCs tested. Furthermore, analysis showed that phenylalanine, threonine, leucine, and lysine contents were the highest among the essential amino acids. Elemental analysis showed that SW amendments contributed to increasing the phosphorus (P) and potassium (K) content in mushrooms harvested. However, SCs containing SW had substantially higher heavy metals (Al and As) content. Our results indicate that SW from anaerobic digestion of poultry litter could increase fruit body yield and nutritional content in P. ostreatus when added at low concentrations (10%, w/w) in cultivation substrates.