Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

Volumes:
Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v14.i3.40
pages 271-284

Isolation and Purification of Polysaccharides with Anti-tumor Activity from Pholiota adiposa (Batsch) P. Kumm. (Higher Basidiomycetes)

Qingxiu Hu
Institute of agricultral source and regional planning research, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193
Hexiang Wang
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
Tzi Bun Ng
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

SINOPSIS

Pholiota adiposa is a mushroom with excellent medicinal and nutritional properties. After culture in fermentation medium, Ph. Adiposa mycelia were filtered, lyophilized, and powdered. A crude polysaccharide (PAP) of Ph. Adiposa was prepared from the mycelial powder with hot water, centrifuged, and the resulting supernatant lyophilized. PAP was fractionated by 30%, 60%, and 80% ethanol precipitation steps to yield PAP30, PAP60, and PAP80. Subsequently PAP30-1 and PAP30-2, PAP60-1 and PAP60-2, and PAP80-1 and PAP80-2 were isolated from PAP30, PAP60, and PAP80, respectively, by ion-exchange chromatography on a DEAE-Sepharose column. Polysaccharide content increased from 43.8% in PAP to 50.54%~73.19% in PAP30-1~PAP80-2. The protein content was 4.92% at minimum in these polysaccharide products. In order to identify the chemical composition, the six polysaccharides (PAP30-1, PAP30-2, PAP60-1, PAP60-2, PAP80-1, and PAP80-2) were further purified by gel filtration on Sephacryl S-100-500. Finally, three water-soluble polysaccharides (PAP30-2a, PAP60-2b, and PAP80-2a) were obtained. HPLC analysis revealed that PAP30-2a, PAP60-2b, and PAP80-2a exhibited a molecular weight of 6.6 × 105 Da, 8.4 × 103 Da, and 3.5 × 103 Da, respectively. The glucose content in PAP80-2a, PAP60-2b, and PAP30-2a was 57.8%, 72.7%, and 68.9%, respectively. PAP30-2a, PAP60-2b, and PAP80-2a demonstrated significant differences in anti-tumor activity in mice. PAP80-2a is the optimal bioactive constituent with anti-tumor and T-lymphocyte proliferation stimulating effects.


Articles with similar content:

A Lectin Purified from Blood Red Bracket Mushroom, Pycnoporus sanguineus (Agaricomycetidae), Mycelium Displayed Affinity Toward Bovine Transferrin
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 1
Valeria Grazu, Jesus Martinez de la Fuente, María Pía Cerdeiras, Laura Franco Fraguas, Maria Moros, Silvana Alborés
Production, Purfication, Molecular Characterization and Bioactivities of Exopolysaccharides Produced by the Wine Cap Culinary-Medicinal Mushroom, Stropharia rugosoannulata 2# (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 4
Peixin He, Jizhong Wang, Chunping Xu, Lujing Geng
Extraction Optimization, Composition Analysis, and Antioxidation Evaluation of Polysaccharides from White Jelly Mushroom, Tremella fuciformis (Tremellomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 12
Yu Zou, Xiyan Hou
Isolation and Characterization of a Glucan-Type Polysaccharide from the Red Pine Mushroom, Lactarius deliciosus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 6
Ana Villares
Chemical Characterization and Structure of Exopolysaccharides from Submerged Culture of New Medicinal Mushroom from China, Phellinus mori (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 1
Feng Peng, Bao-Kai Cui, Chunlei Cao