Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

Volumen 22, 2020 Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v10.i3.90
pages 279-292

The Influence of Cultivation Conditions on Mycelial Growth and Exopolysaccharide Production of Culinary-Medicinal Mushroom Pleurotus citrinopileatus Singer (Agaricomycetideae)

Chiu-Yeh Wu
Department of Culinary Arts, Chung Chou University of Science and Technology, Changhua, Taiwan, Republic of China


The effects of submerged culture conditions on the mycelial growth and exopolysaccharide (EPS) production by Pleurotus citrinopileatus in submerged culture were studied. Using the one-factor-at-a-time method, the suitable rotation speed and inoculation density for the mycelial biomass and EPS production were found to be 100 rpm and 10%, respectively. The medium volume, carbon and nitrogen sources were 100 mL fructose and soy peptone for the mycelial growth and 50 mL glucose and peptone for the EPS production. To study the interactions between glucose (1.32−4.68 g/100 mL), peptone (0.32−3.68 g/100 mL), and initial pH (3.32−6.68), the central composite rotatable design and response surface methodologies were used. The trials were performed in 250-mL flasks containing 50 mL of medium under the condition of 25°C and 100 rpm for 14 days. The components were found to be 3.50 g glucose/100 mL, 3.68 g peptone/100 mL, and initial pH 5.0, and the mycelial biomass of 1.34 g dry cell weight/100 mL and EPS of 64.20 mg/100 mL were obtained in submerged cultures, which were higher than those obtained in the basal medium, respectively. In a 5-L stirred-tank bioreactor, maximum mycelial biomass of 1.10 g dry cell weight/100 mL and EPS of 90 mg/100 mL were achieved, and the fermentation time was shortened from 14 days to 10 days under these cultivation conditions.

Articles with similar content: