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International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

Volumes:
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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019030857
pages 583-593

Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes) Polysaccharides Suppress Tacrine-induced Apoptosis by ROS-scavenging and Mitochondrial Pathway in HepG2 Cells

Zhiguo Li
Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China
Junjie Mei
Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China
Liping Jiang
Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China
Chengyan Geng
Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China
Qiujuan Li
Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China
Xiaofeng Yao
Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China
Jun Cao
Occupational and Environmental Health Department, Dalian Medical University, No. 9, West Segment of South Lvshun Road, Dalian 116044, China

SINOPSIS

Tacrine is the first drug licensed for the treatment of Alzheimer disease. Unfortunately, reversible hepatotoxicity limits its clinical use. In our previous study, we found that tacrine induced apoptosis in HepG2 cells by reactive oxygen species (ROS) formation and mitochondria dysfunction. Inonotus obliquus is a mushroom traditionally used as a folk medicine in Asia. In this study, the possible protective effect of polysaccharides from I. obliquus was investigated. The results showed that I. obliquus polysaccharides (IOP) reduced tacrine-induced apoptosis in HepG2 cells. Inhibition of tacrine-induced ROS generation, 8-OHdG formation in mitochondrial DNA, and loss of the mitochondrial transmembrane potential by IOP were also observed. Furthermore, IOP decreased the cytochrome c release and activation of caspase-3 induced by tacrine. These data suggest that IOP could inhibit tacrine-induced apoptosis in HepG2 cells. The protection is mediated by an antioxidant protective mechanism. Consumption of IOP may be a plausible way to prevent tacrine-induced hepatotoxicity.

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