Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017024762
pages 1123-1133

Lectin from the Late Oyster Mushroom, Hohenbuehelia serotina (Agaricomycetes), and Its Novel Effect as an Adjuvant of the HBV DNA Vaccine

Yingyin Xu
State Key Laboratory for Agro-Biotechnology, Department of Microbiology and Immunology, China Agricultural University, Beijing, People's Republic of China
Shiwen Chen
State Key Laboratory for Agro-Biotechnology, Department of Microbiology and Immunology, China Agricultural University, Beijing, People's Republic of China
Qinghong Liu
State Key Laboratory for Agro-Biotechnology, Department of Microbiology and Immunology, China Agricultural University, Beijing, People's Republic of China

SINOPSIS

The biological macromolecule Hohenbuehelia serotina lectin (HSL) was first isolated from dried fruiting bodies of the mushroom H. serotina and identified as a heterodimer with 2 subunits of the same molecular weight (15.6 kDa) but different isoelectric points. Lactose and d-galactose inhibited the hemagglutinating activity of HSL, whereas mental ions Mn2+ and Ca2+ could stimulate its hemagglutination. The HSL hemagglutinating activity was stable for 1 hour in NaOH and HCl solutions up to a concentration of 12.5 or 6 mmol/L. In addition, HSL was stable up to 50°C for 30 minutes; its hemagglutinating activity was halved at 60°C and totally inactivated above 90°C. HSL (10 μg) as an immune adjuvant co-inoculated with the proVAX/S2 vaccine enhanced the level of hepatitis B surface antigen in C57BL/6 mice, induced a high level of T-cell proliferation, and induced the expression of IFN of CD4+ cells. We further illustrate that HSL, as an adjuvant upregulating the expression of major histocompatibility complex II, contributed to the maturation of dendritic cells. As the first lectin isolated from H. serotina, HSL is a potential adjuvant to chronic hepatitis B virus DNA vaccines and lays a foundation for the prevention of HBV infection.


Articles with similar content:

Antitumor Effect of Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.: Fr.) P. Kumm., Derived Protein Fraction on Tumor-Bearing Mice Models
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 5
Mohitosh Mandal, Birendra Behera, Sujit Kumar Bhutia, Swatilekha Maiti, Tapas K Maiti, Sanjaya Kumar Mallick
Bioactive Components of Ganoderma lucidum (W.Curt.:Fr.) Lloyd Can Induce Apoptosis of Tumor Cells
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Martin Zimmerman-Kordmann, Jing-Song Zhang, Hua Fan, Werner Reutter, Qingjiu Tang
Activation of NADPH Oxidase by β-Glucan from Phellinus baumii (Agaricomycetes) in RAW 264.7 Cells
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 11
Ha Won Kim, Dong Hee Lee, Soo Kyung Sung, Sainkhuu Batbayar
Comparative Study of the Hemagglutinating Activity of Lectins Isolated from Different Developmental Stages of Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.: Fr.) Kumm. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Elene Davitashvili, Ekaterine Kapanadze, Eva Kachlishvili, Eka Metreveli, Vladimir I. Elisashvili
Eupatilin, a Pharmacologically Active Flavone Derived from Artemisia Plants, Induces Apoptosis in Human Gastric Cancer (AGS) Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.24, 2005, issue 4
Tae Young Oh, Min-Jung Kim, Do-Hee Kim, Hye-Kyung Na, Young-Joon Surh, Chang-Yell Shin