Suscripción a Biblioteca: Guest
Portal Digitalde Biblioteca Digital eLibros Revistas Referencias y Libros de Ponencias Colecciones
International Journal of Medicinal Mushrooms
Factor de Impacto: 1.423 Factor de Impacto de 5 años: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

Volumes:
Volumen 21, 2019 Volumen 20, 2018 Volumen 19, 2017 Volumen 18, 2016 Volumen 17, 2015 Volumen 16, 2014 Volumen 15, 2013 Volumen 14, 2012 Volumen 13, 2011 Volumen 12, 2010 Volumen 11, 2009 Volumen 10, 2008 Volumen 9, 2007 Volumen 8, 2006 Volumen 7, 2005 Volumen 6, 2004 Volumen 5, 2003 Volumen 4, 2002 Volumen 3, 2001 Volumen 2, 2000 Volumen 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018026571
pages 647-655

Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium

Chiu-Yeh Wu
Department of Culinary Arts, Chung Chou University of Science and Technology, Changhua, Taiwan, Republic of China
Zeng-Chin Liang
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan

SINOPSIS

We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus. When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass. These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column. Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy. Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan. Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium. By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.


Articles with similar content:

Production of Biomass, Polysaccharides, and Ganoderic Acid using Non-conventional Carbon Sources under Submerged Culture of the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 2
Diego F. Rojas, Paola A. Zapata, Lucia Atehortua
The Influence of the Cultivation Conditions on the Chemical Composition of Medicinal Mushrooms Pleurotus ostreatus (Jacq.: Fr.) Kumm. and Lentinus edodes (Berk.) Sing.
International Journal of Medicinal Mushrooms, Vol.1, 1999, issue 2
Valentina G. Babitskaya, Victor V. Scherba, Valentina I. Fomina, Vycheslav V. Truchonovec, Nina A. Bisko, Nadezda Yu. Mitropolskaya
Characterization and Antioxidant Activity of an Exopolysaccharide Produced by Rigidoporus microporus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 4
Ran Panpan, Yan Wu, Xuewei Jia, Shuai Liu, Chunping Xu, Lili Qu
Antioxidant Activity of Submerged Cultured Mycelium Extracts of Higher Basidiomycetes Mushrooms
International Journal of Medicinal Mushrooms, Vol.9, 2007, issue 2
Abraham Reznick, Mikheil D. Asatiani, Vladimir I. Elisashvili, Eviatar D. Nevo
Species of Genus Ganoderma (Agaricomycetes) Fermentation Broth: A Novel Antioxidant and Antimicrobial Agent
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 5
Mirjana Stajić, Jelena Vukojević, Marijana Kosanic, Jasmina Ćilerdžić, Branislav Ranković