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International Journal of Medicinal Mushrooms
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ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v1.i1.70
pages 89-92

Flammulin Purified from the Fruit Bodies of Flammulina velutipes (Curt.:Fr.) P.Karst.

Hua Zhang
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Feng Gong
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
Yongjun Feng
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Changkai Zhang
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China

SINOPSIS

Flammulin, an antitumor substance, was purified from the aqueous extract of basidiomes of Flammulina velutipes (Curt.: Fr.) P.Karst. It was judged homogenous by SDS-polyacrylamide gel electrophoresis. The purification procedure included ammonium sulfate precipitation, ethanol fractionation, DEAE-Sephadex anion-exchange chromatography, crystallization, and recrystallization. Flammulin was crystallized with microdialysis against a polyethylene glycol sodium phosphate buffer. The crystals were rhombohedron-shaped and easy to melt when the crystallization conditions were changed. With a molecular mass of 23,700, flammulin was characterized by its lack of Met. Amino acid composition analysis showed that the numbers of acidic amino acids are more than that of basic amino acids, but the isoelectric point detected by analytical isoelectric focusing was at pH8.9.


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