RT Journal Article
ID 1252f7777c6f69b7
A1 Ye, Libin
A1 Zheng, Hongjian 
A1 Zhang, Zhong 
A1 Yang, Yan
T1 Preparative Isolation of 5 Antioxidant Constituents from the Medicinal Mushroom <i>Phellinus baumii</i> (Agaricomycetes) by High-Speed Countercurrent Chromatography and Preparative High-Performance Liquid Chromatography
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2017
FD 2017-06-07
VO 19
IS 4
SP 319
OP 326
K1 antioxidant
K1 HSCCC
K1 isolation
K1 medicinal mushrooms
K1 <i>Phellinus baumii</i>
K1 radical scavenger
AB Four extract fractions from the fruiting bodies of <i>Phellinus baumii</i> were acquired. Preliminary investigation of radical scavenging ability showed that the ethyl acetate extract exhibited the strongest antioxidant activity. To gain insight into the antioxidant mechanism, 5 antioxidant constituents were prepared from the ethyl acetate extract using high-speed countercurrent chromatography with a solvent system consisting of n-hexane, ethyl acetate, methanol, and water (0.8:3:1.5:2.5, v/v/v/v), and preparative high-performance liquid chromatography. Electrospray ionization mass spectrometry and nuclear magnetic resonance spectra were used to analyze the structures of these compounds and revealed that they were caffeic acid (compound 1), inoscavin A (compound 2), 3,4-dihydroxy benzaldehyde (compound 3), coumarin (compound 4), and naringenin (compound 5). Compound 2 showed strong
antioxidant activity, and compounds 1, 3, and 5 showed moderate antioxidant activity. Thus, a more clearly and usefully scientific background is provided herein for the development of relative functional foods or biomedical products using <i>Ph. baumii </i>as the raw material.

PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7e712f4f2518a7dc,1252f7777c6f69b7.html