%0 Journal Article %A Muszynska, Bozena %A Grzywacz, Agata %A Kala, Katarzyna %A Gdula-Argasinska, Joanna %D 2018 %I Begell House %K a-linolenic acid, Agaricus bisporus, anti-inflammatory activity, Caco-2 cells, medicinal mushrooms, mycelial in vitro culture %N 2 %P 129-139 %R 10.1615/IntJMedMushrooms.2018025408 %T Anti-Inflammatory Potential of In Vitro Cultures of the White Button Mushroom, Agaricus bisporus (Agaricomycetes), in Caco-2 Cells %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,6866212879aab21d,1440bed9007f0689.html %V 20 %X Agaricus bisporus (white button mushroom) is one of the most popular culinary-medicinal mushrooms worldwide. This species has for decades been the subject of numerous scientific studies. The aim of this study was to examine the pro- or anti-inflammatory properties of A. bisporus and biomass extracts from in vitro cultures growing in Oddoux medium enriched with α-linolenic acid in colon epithelial Caco-2 cells activated with lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-α. Incubation of Caco-2 cells with A. bisporus extracts resulted in decreased expression of cyclooxygenase-2 and prostaglandin F2α receptor compared with the LPS- and/or TNF-α-activated cells, whereas the expression of nuclear factor (erythroid-derived 2)-like 2 increased after incubation. Interleukin-6 level decreased significantly in Caco-2 cells after supplementation with mushroom extracts. The amounts of monoun-saturated and polyunsaturated fatty acids differed significantly in Caco-2 cell membranes after supplementation with A. bisporus extracts. Our findings suggest the presence of anti-inflammatory and antioxidant properties of A. bisporus biomass extracts from in vitro cultures. %8 2018-04-04