RT Journal Article ID 313602623bcb1696 A1 Abidin, Mohamad Hamdi Zainal A1 Abdullah, Noorlidah A1 Abidin, Nurhayati Zainal T1 Protective Effect of Antioxidant Extracts from Grey Oyster Mushroom, Pleurotus pulmonarius (Agaricomycetes), Against Human Low-Density Lipoprotein Oxidation and Aortic Endothelial Cell Damage JF International Journal of Medicinal Mushrooms JO IJM YR 2016 FD 2016-04-18 VO 18 IS 2 SP 109 OP 121 K1 medicinal mushrooms K1 oxidative stress K1 antioxidant K1 functional food K1 LDL oxidation K1 human aortic endothelial membrane damage AB This study evaluated the in vitro antioxidant capacities of extracts from Pleurotus pulmonarius via Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, metal chelating, cupric ion reducing antioxidant capacity, and lipid peroxidation inhibition assays. Extract compositions were determined by phenol−sulfuric acid; Coomassie Plus (Bradford) protein; Spectroquant zinc, copper, and manganese test assays; and liquid chromatography−tandem mass spectrometry (LC/MS/MS) and gas chromatography−mass spectrometry (GC/MS). Methanol-dichloromethane extract, water fraction, hot water, aqueous extract and hexane fraction exhibited the most potent extracts in the antioxidant activities. LC/MS/MS and GC/MS showed that the extracts contained ergothioneine, ergosterol, flavonoid, and phenolic compounds. The selected potent extracts were evaluated for their inhibitory effect against oxidation of human low-density lipoproteins and protective effects against hydrogen peroxide−induced cytotoxic injury in human aortic endothelial cells. The crude aqueous extract was deemed most potent for the prevention of human low-density lipoprotein oxidation and endothelial membrane damage. Ergothioneine might be the compound responsible for the activities, as supported by previous reports. Thus, P. pulmonarius may be a valuable antioxidant ingredient in functional foods or nutraceuticals. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,3b344c674f08ce4b,313602623bcb1696.html