%0 Journal Article %A Camerin, Monica %A Soncin, Marina %A Piccinni, Ester %A Irato, Paola %A Boldrin, Francesco %A Guidolin, Laura %A Jori, Giulio %A Santovito, Gianfranco %D 2011 %I Begell House %K photosensitization, photodamage, tumor cells, RNA microarray %N 3 %P 227-236 %R 10.1615/ForumImmunDisTher.2011004397 %T Gene Expression Profile in Murine 3T3 Fibroblasts Photosensitized by a Tetracationic Porphyrin %U https://www.dl.begellhouse.com/journals/2c6306423483e001,7f17fb734918f4c8,3186af071ca1c0a4.html %V 2 %X The interest regarding the study of cell death in the context of photodynamic therapy (PDT) is quite vigorous. The phenomena of apoptosis, autophagy, and necrosis have been observed thereby, and the specific results depend on the treatment parameters and cell line. To identify key genes expressed in response to photosensitization after short-term exposure to porphyrins and visible light, a study of the transcriptome of murine 3T3 fibroblast cell cultures was performed using the microarray approach. We studied the effect induced by a tetracationic mesosubstituted porphyrin (C12) in this cell line, employing different incubation times and photosensitizer concentrations. A limited mortality (approximately 5%) was obtained by irradiating cells (1 min, 10 mW/cm2) incubated with 0.1 μM C12 for 1 h, hence these samples are suitable for studying initial gene expression. The analysis of microarray data produced the identification of 521 differentially expressed genes in treated cells with respect to controls, 57 of which were overexpressed. Among these, the 25% are involved in cell proliferation and differentiation processes. Notably, one of the overexpressed genes, the tumour suppressor candidate 1 (tusc 1), was expressed 3-fold more in photosensitized fibroblasts. %8 2012-02-01