%0 Journal Article %A Hida, Toshie H. %A Kawaminami, Hiromi %A Ishibashi, Ken-ichi %A Miura, Noriko N. %A Adachi, Yoshiyuki %A Ohno, Naohito %D 2013 %I Begell House %K medicinal mushrooms, β-glucan, chlorpromazine, cytokine production, Sparassis crispa, interferon gamma, interleukin, lymphocyte function-associated antigen 1, tumor necrosis factor-α %N 6 %P 525-538 %R 10.1615/IntJMedMushr.v15.i6.20 %T Oral Administration of Soluble β-Glucan Preparation from the Cauliflower Mushroom, Sparassis crispa (Higher Basidiomycetes) Modulated Cytokine Production in Mice %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,034eeb045436a171,38e14a7f5cf8e5d0.html %V 15 %X Soluble β-glucan preparation from the cold NaOH extract of Sparassis crispa (SCG) is a six-branched 1,3-β-D-glucan that is a major cell-wall structural component in fungi. Leukocytes from DBA/2 mice are highly sensitive to SCG, producing cytokines in vitro. We previously reported that the intraperitoneal (i.p.) administration of β-glucan decreased cytokine induction by SCG in vitro in DBA/2 mice. In this study, we examined the effects of the oral (p.o.) administration of polysaccharide fractions extracted from S. crispa, using hot water (SCHWE), a β-glucan from S. crispa, to DBA/2 mice on cytokine induction by SCG in the spleen in vitro. The level of induction of IFN-γ and GM-CSF by SCG was significantly increased in SCHWE-treated mice. This activity was more clearly observed when chlorpromazine was administered as a pretreatment in SCHWE-treated mice. The production of GM-CSF, IFN-γ, and IL-6 by immune cells in Peyer's patches was higher in SCHWE-treated mice than in control mice. These results suggest that orally administered β-glucan may modulate cytokine induction by SCG in the spleen through the activation of Peyer's patches.#947; and GM-CSF by SCG was significantly increased in SCHWE-treated mice. This activity was more clearly observed when chlorpromazine was administered as a pretreatment in SCHWE-treated mice. The production of GM-CSF, IFN- %8 2013-11-15