RT Journal Article
ID 393678f326c6e405
A1 Feng, Ting
A1 Jia, Wei
A1 Wang, Wenhan
A1 Lin, Chi-Chung
A1 Fan, Hua
A1 Zhang, Jingsong
A1 Bao, Hai-Ying
T1 Structural Characterization and Immunological Activities of a Novel Water-Soluble Polysaccharide from the Fruiting Bodies of Culinary-Medicinal Winter Mushroom, Flammulina velutipes (Agaricomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2016
FD 2016-11-18
VO 18
IS 9
SP 807
OP 819
K1 Flammulina velutipes
K1 immunological activities
K1 medicinal mushrooms
K1 polysaccharides
K1 purification
AB A water-soluble polysaccharide, designated FVPA2, was isolated from the fruiting bodies of Flammulina velutipes using DEAE Sepharose Fast Flow and gel-permeation chromatography. Its structure was elucidated by monosaccharide composition and methylation analysis, ultraviolet, Fourier transform infrared spectrometry, and nuclear magnetic resonance spectroscopy. Results showed that FVPA2 was a homogeneous heteropolysaccharide containing galactose, fucose, and mannose in a molar ratio of 5:1:1. High-performance liquid chromatography indicated its molecular weight as 3.4 × 104 Da. FVPA2 also has a repeating unit. In vitro immunomodulatory studies showed that Raw264.7 cells were stimulated to secret nitric oxide upon administration of 200−500 µg/mL FVPA2. FVPA2 also stimulated the proliferation of mouse spleen lymphocytes and B lymphocytes.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2e89624a4c27b9d4,393678f326c6e405.html