RT Journal Article ID 393678f326c6e405 A1 Feng, Ting A1 Jia, Wei A1 Wang, Wenhan A1 Lin, Chi-Chung A1 Fan, Hua A1 Zhang, Jingsong A1 Bao, Hai-Ying T1 Structural Characterization and Immunological Activities of a Novel Water-Soluble Polysaccharide from the Fruiting Bodies of Culinary-Medicinal Winter Mushroom, Flammulina velutipes (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2016 FD 2016-11-18 VO 18 IS 9 SP 807 OP 819 K1 Flammulina velutipes K1 immunological activities K1 medicinal mushrooms K1 polysaccharides K1 purification AB A water-soluble polysaccharide, designated FVPA2, was isolated from the fruiting bodies of Flammulina velutipes using DEAE Sepharose Fast Flow and gel-permeation chromatography. Its structure was elucidated by monosaccharide composition and methylation analysis, ultraviolet, Fourier transform infrared spectrometry, and nuclear magnetic resonance spectroscopy. Results showed that FVPA2 was a homogeneous heteropolysaccharide containing galactose, fucose, and mannose in a molar ratio of 5:1:1. High-performance liquid chromatography indicated its molecular weight as 3.4 × 104 Da. FVPA2 also has a repeating unit. In vitro immunomodulatory studies showed that Raw264.7 cells were stimulated to secret nitric oxide upon administration of 200−500 µg/mL FVPA2. FVPA2 also stimulated the proliferation of mouse spleen lymphocytes and B lymphocytes. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2e89624a4c27b9d4,393678f326c6e405.html