RT Journal Article
ID 4baa0270121fbe52
A1 Zhang, Chaohui
A1 Huang, Tao
A1 Shen, Chaohui
A1 Wang, Xiaoting
A1 Qi, Yuancheng
A1 Shen, Jinwen
A1 Song, Andong
A1 Qiu, Liyou
A1 Ai, Yuncan
T1 Downregulation of Ethylene Production Increases Mycelial Growth and Primordia Formation in the Button Culinary-Medicinal Mushroom, Agaricus bisporus (Agaricomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2016
FD 2017-01-09
VO 18
IS 12
SP 1131
OP 1140
K1 1-aminocyclopropane-1-carboxylate synthase
K1 1-aminocyclopropane-1-carboxylate oxidase
K1 Agaricus bisporus
K1 ethylene
K1 medicinal and edible mushrooms
K1 regulation
AB Ethylene biosynthesis and function in Agaricus bisporus (the button mushroom) remain uncertain. The enzyme activities of 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) and ACC oxidase (ACO) were detectable in A. bisporus AS2796 and inhibited by α-aminooxyacetic acid and Co2+. We cloned and sequenced 2 ACS genes (Ab-ACS1 and Ab-ACS2) and 1 ACO gene (Ab-ACO) from the mushroom strain. Ab-ACS1 and Ab-ACS2 demonstrated low amino acid sequence similarity. Ab-ACO demonstrated an amino acid sequence completely identical to that of ACO1_AGABI from A. bisporus. Antisense ACO significantly reduced ACO gene expression level, ACO enzyme activity, and ethylene production in the mushroom transformants. The transformants grew faster than the wild-type strain in sterilized compost and normally formed primordia when cultivated in sterilized compost with the sterilized casing vermiculite, but the wild-type strain did not. Our results show that ethylene is synthesized in button mushrooms via the ACC pathway. Ethylene inhibited button mushroom mycelial growth and development.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,57162b26264a3b98,4baa0270121fbe52.html