RT Journal Article ID 4baa0270121fbe52 A1 Zhang, Chaohui A1 Huang, Tao A1 Shen, Chaohui A1 Wang, Xiaoting A1 Qi, Yuancheng A1 Shen, Jinwen A1 Song, Andong A1 Qiu, Liyou A1 Ai, Yuncan T1 Downregulation of Ethylene Production Increases Mycelial Growth and Primordia Formation in the Button Culinary-Medicinal Mushroom, Agaricus bisporus (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2016 FD 2017-01-09 VO 18 IS 12 SP 1131 OP 1140 K1 1-aminocyclopropane-1-carboxylate synthase K1 1-aminocyclopropane-1-carboxylate oxidase K1 Agaricus bisporus K1 ethylene K1 medicinal and edible mushrooms K1 regulation AB Ethylene biosynthesis and function in Agaricus bisporus (the button mushroom) remain uncertain. The enzyme activities of 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) and ACC oxidase (ACO) were detectable in A. bisporus AS2796 and inhibited by α-aminooxyacetic acid and Co2+. We cloned and sequenced 2 ACS genes (Ab-ACS1 and Ab-ACS2) and 1 ACO gene (Ab-ACO) from the mushroom strain. Ab-ACS1 and Ab-ACS2 demonstrated low amino acid sequence similarity. Ab-ACO demonstrated an amino acid sequence completely identical to that of ACO1_AGABI from A. bisporus. Antisense ACO significantly reduced ACO gene expression level, ACO enzyme activity, and ethylene production in the mushroom transformants. The transformants grew faster than the wild-type strain in sterilized compost and normally formed primordia when cultivated in sterilized compost with the sterilized casing vermiculite, but the wild-type strain did not. Our results show that ethylene is synthesized in button mushrooms via the ACC pathway. Ethylene inhibited button mushroom mycelial growth and development. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,57162b26264a3b98,4baa0270121fbe52.html