RT Journal Article ID 5e7d673d20dfe743 A1 He, Peixin A1 Yu, Miao A1 Cai, Yingli A1 Liu, Wei A1 Wang, Wensheng A1 Wang, Shaohua A1 Li, Jing T1 Effect of Aging on Culture and Cultivation of the Culinary-Medicinal Mushrooms Morchella importuna and M. sextelata (Ascomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2019 FD 2019-12-11 VO 21 IS 11 SP 1089 OP 1098 K1 field cultivation K1 medicinal mushrooms K1 Morchella importuna K1 M. sextelata K1 morel K1 mycelial growth K1 oxidative stress K1 successive culturing AB The prominent problem of "uncertainty" has been frustrating morel farming since the commercial production of Morchella mushrooms was realized in 2012 in China. Spawn aging may be the main underlying reason. In this paper, aging in cultivated strains of M. importuna T4 and M. sextelata T6 was achieved by successive subculturing. Oxidative stress, mycelial growth rate, biomass, sclerotial formation, pigmentation, and yield of different subcultures were determined. The results suggested that M. importuna T4 and M. sextelata T6 exhibited systemic senescence manifested as 24 and 17 subcultures and lifespan of 3048 and 2040 h, respectively. Aging showed a close relevance to oxidative stress. Regression analysis revealed a strong positive correlation between time of subculturing (aging) and lipid peroxidation (oxidative stress) in both morels. In addition, pigmentation tended to increase, while the number of sclerotia tended to decrease, with the rise of subculturing times in both morels. Moreover, the mycelial growth rate and biomass of the last two subcultures were significantly lower than those of others, indicating that cultural characteristics may be used as signs of seriously aging culture. Finally, the yield of subcultures was significantly lower than that of the original strains in artificial cultivation. Regression analysis showed a strong negative correlation between time of subculturing and yield in two morels. This work will improve the understanding of "uncertainty" and thus be beneficial for stable development of morel farming. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,25f9c61f6dbb0698,5e7d673d20dfe743.html