RT Journal Article
ID 60fa5b987ee94cb2
A1 Vodickova, Anna
A1 Schmiedberger, J. 
T1 Utilization of Reactive Oxygen Species Generated by Electric Discharge in Biomedical Engineering
JF Plasma Medicine
JO PMED
YR 2018
FD 2018-05-09
VO 8
IS 2
SP 147
OP 154
K1 discharge singlet oxygen generator
K1 singlet oxygen
K1 cell lines
K1 oncology
K1 reactive oxygen species
AB The discharge singlet oxygen generator-6 (DSOG-6) is a device generating reactive oxygen and nitrogen species (including singlet oxygen, ozone, atomic oxygen, NO<sub>x</sub>) by a hybrid RF/DC electric discharge. These reactive species are often used in biomedicine. In this study, reactions of selected cell cultures (B16, HT29, and HaCat) with DSOG-6-generated particles were observed and analyzed. Cell cultures were exposed to singlet oxygen or reactive oxygen species generated by a hybrid electric discharge for different time periods. After the exposure, a microscopic analysis of the cytomorphology of cells was performed. Furthermore, cell proliferation of the B16 cell line was investigated by the xCELLigence System (Acea Biosciences Inc., San Diego, CA). Finally, preliminary results of qPCR from B16 exposed to singlet oxygen are presented. The main goal of this study was to evaluate if singlet oxygen/reactive oxygen species generated by DSOG-6 could be used in biomedical application (concretely in oncology and dermatology) and if DSOG-6 could become a new biomedical device. The secondary aim was to observe the dependence of cell lines' behavior on exposure parameters (e.g., time intervals and particles in discharge).



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