%0 Journal Article %A Moldavan, Mykhaylo %A Grodzinskaya, Anna A. %A Storozhuk, Victor M. %D 2002 %I Begell House %N 1 %P 6 %R 10.1615/IntJMedMushr.v4.i1.50 %T Neurotropic Effect of the Edible and Medicinal Mushroom Lentinus edodes (Berk.) Sing. Extracts on the Neurons of Hippocampal Slices in Rats %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2ea43b2e1c72ed1c,6895f4835a6d6f64.html %V 4 %X Effects of Lentinus edodes (Berk.) Sing. extract on the spike activity of 28 pyramidal neurons from the CA1 region of the albino rat hippocampus were studied. In 12 (43%) neurons the extract application resulted in inhibition; in 4 (14%) neurons an insignificant increase in the spike discharge frequency was observed; and 12 (43%) cells showed no response. Latency and duration were as follows: for inhibitory responses (71 ± 14 s, 192 ± 26 s), for excitatory responses (63 ± 19 s, 116 ± 30 s). During washing, 11 neurons showed periodical group spike discharges that occurred on the average for 182 ± 35 s, but in one neuron they lasted for 34 minutes. It was suggested that in most cases the extract action caused a hyperpolarization of neuron membranes that during washing was replaced by a depolarization that was associated with the emergence of group discharges. An antagonist of GABA receptors, bicuculline, and an antagonist of 5-HT2/5-HT1C serotonin receptors, ritanserin, blocked an inhibitory effect of the extract partially or completely. This indicates that GABA or its agonist and substances that may activate serotonin receptors are involved in inhibition of neuron spike responses during the extract application. %8 2002-03-01