RT Journal Article
ID 7a21759075ddedba
A1 Chen, Ti Qiang
A1 Kan, Yong-Jun
A1 Yang, Chi
A1 Wu, Jian-Guo
A1 Wu, Jin-Zhong
T1 Bioactivities and Molecular Characterizations of the Polysaccharide(s) from Ultrasonic-Circulating Extracts of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2020
FD 2020-06-15
VO 22
IS 5
SP 455
OP 466
K1 Ganoderma lucidum
K1 polysaccharide
K1 ultrasonic-circulating extraction
K1 ethanol fractionation
K1 antioxidant activity
K1 hepatoprotective activity
K1 medicinal mushrooms
AB Total polysaccharide (i.e., GLP-UCE) was effectively isolated from the crude polysaccharide extract powder of Ganoderma lucidum by ultrasonic-circulating extraction, which was separated into three fractions (i.e., GLP-U40, GLP-U60, and GLP-U80) by ethanol fractional precipitation. The detection of antioxidant enzymes (GSH-Px, SOD) and oxidation metabolites (MDA, LF); liver function test of ALT, AST, and LDH; and western blot for antioxidant proteins of GSH-Px and MMP-2 showed that the GLP-UCE displayed a favorable in vivo antioxidant and hepatoprotective activities for reducing the oxidative damage in CCl4-hepatopathy SD rats. The GLP-UCE and its fractions were analyzed and compared by Fourier transform infrared (FT-IR), high-performance liquid chromatography, high-performance gel permeation chromatography, and antioxidant activity in vitro assay. These studies revealed that the fraction GLP-U80 exhibited stronger antioxidant activities in vitro than that of GLP-UCE and other fractions (p<< 0.01), which was homogeneous and heteropolysaccharide-rich in β-D-glucose with a weight-average molecular mass (Mw) of 1.05 × 104 g·mol–1 and composed of D-mannose, D-ribose, D-glucose, D-galactose, and L-fucose in a molecular ratio of 1:0.26:8.26:0.64:0.08.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,489762826cea4201,7a21759075ddedba.html