RT Journal Article ID 7a21759075ddedba A1 Chen, Ti Qiang A1 Kan, Yong-Jun A1 Yang, Chi A1 Wu, Jian-Guo A1 Wu, Jin-Zhong T1 Bioactivities and Molecular Characterizations of the Polysaccharide(s) from Ultrasonic-Circulating Extracts of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2020 FD 2020-06-15 VO 22 IS 5 SP 455 OP 466 K1 Ganoderma lucidum K1 polysaccharide K1 ultrasonic-circulating extraction K1 ethanol fractionation K1 antioxidant activity K1 hepatoprotective activity K1 medicinal mushrooms AB Total polysaccharide (i.e., GLP-UCE) was effectively isolated from the crude polysaccharide extract powder of Ganoderma lucidum by ultrasonic-circulating extraction, which was separated into three fractions (i.e., GLP-U40, GLP-U60, and GLP-U80) by ethanol fractional precipitation. The detection of antioxidant enzymes (GSH-Px, SOD) and oxidation metabolites (MDA, LF); liver function test of ALT, AST, and LDH; and western blot for antioxidant proteins of GSH-Px and MMP-2 showed that the GLP-UCE displayed a favorable in vivo antioxidant and hepatoprotective activities for reducing the oxidative damage in CCl4-hepatopathy SD rats. The GLP-UCE and its fractions were analyzed and compared by Fourier transform infrared (FT-IR), high-performance liquid chromatography, high-performance gel permeation chromatography, and antioxidant activity in vitro assay. These studies revealed that the fraction GLP-U80 exhibited stronger antioxidant activities in vitro than that of GLP-UCE and other fractions (p<< 0.01), which was homogeneous and heteropolysaccharide-rich in β-D-glucose with a weight-average molecular mass (Mw) of 1.05 × 104 g·mol–1 and composed of D-mannose, D-ribose, D-glucose, D-galactose, and L-fucose in a molecular ratio of 1:0.26:8.26:0.64:0.08. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,489762826cea4201,7a21759075ddedba.html