RT Journal Article
ID 7a3f4ae051745a0e
A1 Zhu, Lina
A1 Luo, Xi
A1 Tang, Qingjiu
A1 Liu, Yanfang
A1 Zhou, Shuai
A1 Yang, Yan
A1 Zhang, Jingsong
T1 Isolation, Purification, and Immunological Activities of a Low-Molecular-Weight Polysaccharide from the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2013
FD 2013-06-13
VO 15
IS 4
SP 407
OP 414
K1 medicinal mushrooms
K1 Ganoderma lucidum
K1 polysaccharide
K1 isolation
K1 purification
K1 immunological activityi
AB To obtain a low-molecular-weight polysaccharide with immuno-enhancing activity, hot water extract of Ganoderma lucidum fruit bodies was separated by membrane ultrafiltration, anion exchange, and gel filtration chromatography, and the immunological activities of fractions were assessed on the basis of nitric oxide production by RAW 264.7 macrophages. A novel polysaccharide (TB3-2-2) was successfully isolated and purified. TB3-2-2 is a homogeneous polysaccharide, with a relative molecular weight of 5.11 × 103 Da, identified by high-performance liquid chromatography and was composed of galactose and glucose in a molar ratio of 2:3 determined by high-performance anion exchange chromatography. TB3-2-2 had a carbohydrate content of 99%, as measured using the phenol-sulfuric acid method. Proliferation of mouse spleen lymphocytes and the expression level of interleukin-6 was significantly increased by TB3-2-2. Results indicate that the low-molecular-weight polysaccharide with immunological activity in G. lucidum is worthy of further research and development.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,3eefb46658ba8153,7a3f4ae051745a0e.html