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Journal of Environmental Pathology, Toxicology and Oncology
Facteur d'impact: 1.241 Facteur d'impact sur 5 ans: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimer: 0731-8898
ISSN En ligne: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v31.i2.50
pages 131-141

Hepatoprotective Efficacy of Sharbat-e-Deenar Against Carbon Tetrachloride−Induced Liver Damage

Arvind Shakya
Indira Gandhi National Open University
Monika Sharma
Department of Bioscience and Biotechnology, Banasthali Vidyapith (Women's University), Rajasthan, India
Neetu Sharma
Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior, India
Sadhana Shrivastava
Reproductive Biology and Toxicology Labora¬tory, UNESCO Satellite Center of Trace Element Research, School of Studies in Zoology, Jiwaji University, Gwalior 474011, India
Sangeeta Shukla
School of Studies in Zoology, Jiwaji University, Gwalior 474011, India

RÉSUMÉ

This study was undertaken to evaluate the antioxidant and hepatoprotective efficacy of Sharbat-e-Deenar (SD) against carbon tetrachloride (CCl4)−induced hepatic damage in a rat model. The antioxidant activity of SD was estimated by DPPH assay, H2O2 assay, and total phenolic contents. SD therapy at doses of 1, 2, and 4 mL/kg, orally, was administered after CCl4 intoxication (1.5 mL/kg, intraperitoneally for 48 hours) in experimental animals. Hexobarbitone sleep time and bromosulfophthalein retention time also were determined against CCl4-induced liver damage. Exposure to CCl4 in experimental animals showed biochemical and histopathological deterioration in the liver. Treatment with SD showed a marked protection on biochemical parameters, that is, alanine transaminase, aspartate transaminase, albumin, and urea. SD therapy exhibited a protective effect by restoring the level of lipid peroxidation−reduced glutathione, adenosine triphosphate, and glucose-6-phosphatase (G-6-Pase). Treatment with SD significantly recovered the level of hexobarbitone sleep time and bromosulfophthalein retention time. DPPH and H2O2 assays showed antioxidant properties in a dose-dependent manner. Histopathological observations of liver sections showed recovery in liver architecture after SD treatment. These results indicate that SD exerts a protective effect on CCl4-induced hepatotoxicity, which may be due to its antioxidant properties.