Publication de 4 numéros par an
ISSN Imprimer: 0731-8898
ISSN En ligne: 2162-6537
Indexed in
Gene Profiling of Normal Human Bronchial Epithelial Cells in Response to Asbestos and Benzo(a)pyrene diol epoxide (BPDE)
RÉSUMÉ
Asbestos and benzo(a)pyrene diol epoxide (BPDE) are pulmonary carcinogens with synergistic interaction in causing lung cancer. We used Affymetrix microarrays to study gene modulation in vitro using normal human bronchial epithelial cells exposed to chrysotile asbestos and/or BPDE for 4 or 24 h. Linear models were used to compare treated cells to controls at each time point to identify statistically significant up- or downregulation of genes. Profiles of genes regulated by chrysotile were dominated by cytokines, growth factors, and DNA damage. Profiles of genes with BPDE and chrysotile regulation were correlated with proliferation, DNA damage recognition and nucleotide-excision repair, cytokines, and apoptosis. Chemokines, growth-regulated oncogene-alpha (Gro-α, CXCL-1), and IL-8, were significantly increased, and these had previously been observed in bronchoalveolar lavage from asbestos workers or in animal models. Interestingly, the Hermansky-Pudlak gene, which is mutated in an autosomal recessive form of pulmonary fibrosis, was downregulated threefold by BPDE at 4 h. This is an interesting example of gene (Hermansky-Pudlak syndrome) and environment (BPDE) interaction. Transcription factors, including activating transcription factor 3 and Cbp/p300-interacting transactivator, were upregulated by chrysotile. Real Time PCR for IL-8, ATF-3, GADD45B, CXC Ligand 1, and CTGF compared to GAPDH validated microarray findings at 24 h. These in vitro findings in NHBE cells model environment-gene interaction for asbestos and BPDE, highlighting effects of inflammation, fibrosis, proliferation, and DNA damage recognition and repair.
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Huang Sarah X. L., Jaurand Marie-Claude, Kamp David W., Whysner John, Hei Tom K., Role of Mutagenicity in Asbestos Fiber-Induced Carcinogenicity and Other Diseases, Journal of Toxicology and Environmental Health, Part B, 14, 1-4, 2011. Crossref
-
Yauk Carole Lyn, Jackson Kelly, Malowany Morie, Williams Andrew, Lack of change in microRNA expression in adult mouse liver following treatment with benzo(a)pyrene despite robust mRNA transcriptional response, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 722, 2, 2011. Crossref
-
Hillegass Jedd M., Shukla Arti, MacPherson Maximilian B., Bond Jeffrey P., Steele Chad, Mossman Brooke T., Utilization of Gene Profiling and Proteomics to Determine Mineral Pathogenicity in a Human Mesothelial Cell Line (LP9/TERT-1), Journal of Toxicology and Environmental Health, Part A, 73, 5-6, 2010. Crossref
-
Chen Wenshu, Xu Xiuling, Bai Lang, Padilla Mabel T., Gott Katherine M., Leng Shuguang, Tellez Carmen S., Wilder Julie A., Belinsky Steven A., Scott Bobby R., Lin Yong, Low-dose gamma-irradiation inhibits IL-6 secretion from human lung fibroblasts that promotes bronchial epithelial cell transformation by cigarette-smoke carcinogen, Carcinogenesis, 33, 7, 2012. Crossref
-
Dreij Kristian, Rhrissorrakrai Kahn, Gunsalus Kristin C., Geacintov Nicholas E., Scicchitano David A., Benzo[ a ]pyrene diol epoxide stimulates an inflammatory response in normal human lung fibroblasts through a p53 and JNK mediated pathway, Carcinogenesis, 31, 6, 2010. Crossref
-
Lu Xiangyun, Shao Jimin, Li Hongjuan, Yu Yingnian, Early whole-genome transcriptional response induced by benzo[a]pyrene diol epoxide in a normal human cell line, Genomics, 93, 4, 2009. Crossref
-
Øvrevik J., Låg M., Holme J.A., Schwarze P.E., Refsnes M., Cytokine and chemokine expression patterns in lung epithelial cells exposed to components characteristic of particulate air pollution, Toxicology, 259, 1-2, 2009. Crossref
-
Seo Yoo-Na, Lee Yong-Jin, Lee Mi-Young, Differential gene expression by chrysotile in human bronchial epithelial cells, Animal Cells and Systems, 16, 2, 2012. Crossref
-
Bausinger J., Speit G., DNA repair capacity of cultured human lymphocytes exposed to mutagens measured by the comet assay and array expression analysis, Mutagenesis, 30, 6, 2015. Crossref
-
Lu Xiangyun, Shao Jimin, Li Hongjuan, Yu Yingnian, Temporal gene expression changes induced by a low concentration of benzo[a]pyrene diol epoxide in a normal human cell line, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 684, 1-2, 2010. Crossref
-
Rappolee Daniel A., Awonuga Awoniyi O., Puscheck Elizabeth E., Zhou Sichang, Xie Yufen, Benzopyrene and Experimental Stressors Cause Compensatory Differentiation in Placental Trophoblast Stem Cells, Systems Biology in Reproductive Medicine, 56, 2, 2010. Crossref
-
Osgood Ross S., Upham Brad L., Bushel Pierre R., Velmurugan Kalpana, Xiong Ka-Na, Bauer Alison K., Secondhand Smoke-Prevalent Polycyclic Aromatic Hydrocarbon Binary Mixture-Induced Specific Mitogenic and Pro-inflammatory Cell Signaling Events in Lung Epithelial Cells, Toxicological Sciences, 157, 1, 2017. Crossref
-
Zaccaria Kimberly J., McClure Peter R., Using Immunotoxicity Information to Improve Cancer Risk Assessment for Polycyclic Aromatic Hydrocarbon Mixtures, International Journal of Toxicology, 32, 4, 2013. Crossref
-
HELMIG SIMONE, DOPP ELKE, WENZEL SIBYLLE, WALTER DIRK, SCHNEIDER JOACHIM, Induction of altered mRNA expression profiles caused by fibrous and granular dust, Molecular Medicine Reports, 9, 1, 2014. Crossref
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Wu Meiqiong, Liang Gang, Duan Huiling, Yang Xiaofeng, Qin Guohua, Sang Nan, Synergistic effects of sulfur dioxide and polycyclic aromatic hydrocarbons on pulmonary pro-fibrosis via mir-30c-1-3p/ transforming growth factor β type II receptor axis, Chemosphere, 219, 2019. Crossref
-
Uskoković Vuk, Batarni Samir Shariff, Schweicher Julien, King Andrew, Desai Tejal A., Effect of Calcium Phosphate Particle Shape and Size on Their Antibacterial and Osteogenic Activity in the Delivery of Antibiotics in Vitro, ACS Applied Materials & Interfaces, 5, 7, 2013. Crossref
-
Liu Gang, Cheresh Paul, Kamp David W., Molecular Basis of Asbestos-Induced Lung Disease, Annual Review of Pathology: Mechanisms of Disease, 8, 1, 2013. Crossref
-
Korbecki Jan, Maruszewska Agnieszka, Bosiacki Mateusz, Chlubek Dariusz, Baranowska-Bosiacka Irena, The Potential Importance of CXCL1 in the Physiological State and in Noncancer Diseases of the Cardiovascular System, Respiratory System and Skin, International Journal of Molecular Sciences, 24, 1, 2022. Crossref