Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
Journal of Environmental Pathology, Toxicology and Oncology
Facteur d'impact: 1.241 Facteur d'impact sur 5 ans: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Imprimer: 0731-8898
ISSN En ligne: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2016017340
pages 15-27

Characterization of Microcystin-Induced Dualistic Toxic Effects on Primary Rat Hepatocytes

Yi Shuai
Division of Toxicology, Shanghai Municipal Center for Disease Control & Prevention, Shanghai, 200336, China
Dan Lou
Division of Toxicology, Shanghai Municipal Center for Disease Control & Prevention, Shanghai, 200336, China
Jianxun Yin
Shanghai Zhabei District Center for Disease Control and Prevention, Shanghai, 200072, China
Xiaolan Qian
Shanghai Yangpu District Center for Disease Control and Prevention, Shanghai, 200082, China
Yanqin Wang
Division of Toxicology, Shanghai Municipal Center for Disease Control & Prevention, Shanghai, 200336, China
Xinyu Hong
Division of Toxicology, Shanghai Municipal Center for Disease Control & Prevention, Shanghai, 200336, China
Ping Xiao
Division of Toxicology, Shanghai Municipal Center for Disease Control & Prevention, Shanghai, 200336, China
Weijian Zhong
Division of Toxicology, Shanghai Municipal Center for Disease Control & Prevention, Shanghai, 200336, China

RÉSUMÉ

Microcystins (MCs) comprise a group of widely characterized cyclic heptapeptides able to induce a series of liver injuries, including acute liver failure and primary liver cancer. Although the dualistic effects of MCs have been postulated, the specific action mode according to the exposure dosage of MCs remains unknown. In the present study, primarily cultured rat hepatocytes were used to systematically investigate hepatotoxic characteristics of MC-LR (one of the most abundant and toxic MCs variants). Results showed that the dualistic toxicity of MC-LR on hepatocytes is dose dependent. Specifically, MC-LR at a high dose (>10-8 mol/L) induced a significant reduction in cell viability, whereas low-dose MC-LR (<10-8 mol/L) was observed to promote cell proliferation. Oxidative stress measurements showed that reactive oxygen species (ROS) levels undergo a massive and rapid increase in high-dose MC-LR–treated hepatocytes and a mild and slow increase in low-dose MC-LR–treated hepatocytes. These in vitro data suggest that MC-LR is able to exert dualistic toxic effects on hepatocytes through the "two-faced" character of ROS, which causes cell death or even necrosis at high concentrations and promotes cell proliferation exclusively at low or transient concentrations.


Articles with similar content:

Comparative evaluation of the cytotoxic and apoptotic potential of Poecilocerus pictus and Calotropis gigantea
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
Shiney Mary Peter, Bhagyashree P. Hardikar, Caroline Mathen
Oxidative Mechanisms in the Toxicity of Chromium and Cadmium Ions
Journal of Environmental Pathology, Toxicology and Oncology, Vol.20, 2001, issue 2
Ezdihar Hassoun, Debasis Bagchi, Manashi Bagchi, Sidney J. Stohs
Black Tea-Induced Amelioration of Hepatic Oxidative Stress through Antioxidative Activity in EAC-Bearing Mice
Journal of Environmental Pathology, Toxicology and Oncology, Vol.26, 2007, issue 4
Sreya Chattopadhyay, Tanya Das, Arindam Bhattacharyya, Gaurisankar Sa, Lakshmishri Lahiry, Uttam K. Ghosh, Sankar Bhattacharyya, Deba Prasad Mandal
Biochanin A Enhances the Radiotoxicity in Colon Tumor Cells In Vitro
Journal of Environmental Pathology, Toxicology and Oncology, Vol.32, 2013, issue 3
Reeta Tiwari, Abhay Puthli, Kaushala Prasad Mishra
Aniline Derivative-Induced Methemoglobin in Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.24, 2005, issue 1
Harpal Singh, Elissa T. Purnell