Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
Critical Reviews™ in Biomedical Engineering
SJR: 0.207 SNIP: 0.376 CiteScore™: 0.79

ISSN Imprimer: 0278-940X
ISSN En ligne: 1943-619X

Volumes:
Volume 47, 2019 Volume 46, 2018 Volume 45, 2017 Volume 44, 2016 Volume 43, 2015 Volume 42, 2014 Volume 41, 2013 Volume 40, 2012 Volume 39, 2011 Volume 38, 2010 Volume 37, 2009 Volume 36, 2008 Volume 35, 2007 Volume 34, 2006 Volume 33, 2005 Volume 32, 2004 Volume 31, 2003 Volume 30, 2002 Volume 29, 2001 Volume 28, 2000 Volume 27, 1999 Volume 26, 1998 Volume 25, 1997 Volume 24, 1996 Volume 23, 1995

Critical Reviews™ in Biomedical Engineering

DOI: 10.1615/CritRevBiomedEng.2014010448
pages 281-308

Nanoscale Imaging by Superresolution Fluorescence Microscopy and Its Emerging Applications in Biomedical Research

Cristina Bertocchi
Mechanobiology Institute, Singapore
Wah Ing Goh
Mechanobiology Institute, Singapore
Zhen Zhang
Mechanobiology Institute, Singapore
Pakorn Kanchanawong
Mechanobiology Institute, Singapore; Department of Biomedical Engineering, National University of Singapore, Singapore

RÉSUMÉ

Superresolution microscopy, an ensemble of light microscopy methods developed with the aim of surpassing the resolution limit imposed by diffraction, has been at the forefront of imaging technology innovations in recent years. By harnessing advances in fluorophore photophysics, fluorescent protein engineering, optics, and image processing, rapid strides have been made in enhancing imaging resolution via 3 major approaches: structured illumination microscopy, stimulated emission depletion microscopy, and single-molecule localization microscopy. From a diffraction-limited resolution of ~250 nm, an improvement of more than an order of magnitude down to ~10 nm can now be attained, converging upon the size scale of the macromolecular building blocks of cells. This opens up the possibility of direct visualization of molecular-scale architecture and interactions of specific proteins in biological structures that are important to health and disease. Here, theoretical foundations and practical considerations of superresolution microscopy in 2- and 3-dimensional imaging are discussed, along with their recent applications in addressing biological questions.


Articles with similar content:

Imaging of DNA and Protein−DNA Complexes with Atomic Force Microscopy
Critical Reviews™ in Eukaryotic Gene Expression, Vol.26, 2016, issue 1
Luda S. Shlyakhtenko, Yuri L. Lyubchenko
Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part B− Structuromics
Critical Reviews™ in Eukaryotic Gene Expression, Vol.24, 2014, issue 3
Lenka Stefancikova, Marie Davidkova, Abin Biswas, Jan Sevcik, Michal Hofer, Anna Michaelidesova, Iva Falkova, Emilie Lukasova, Michael Hausmann, Alena Bacikova, Zdenek Kleibl, Alla Boreyko, Pavel Matula, Jana Vachelova, Stanislav Kozubek, Evgeny Krasavin, Martin Falk, Lucie Jezkova, Georg Hildenbrand
Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: PART A−Radiomics
Critical Reviews™ in Eukaryotic Gene Expression, Vol.24, 2014, issue 3
Lenka Stefancikova, Marie Davidkova, Abin Biswas, Jan Sevcik, Michal Hofer, Anna Michaelidesova, Iva Falkova, Emilie Lukasova, Michael Hausmann, Alena Bacikova, Zdenek Kleibl, Alla Boreyko, Pavel Matula, Jana Vachelova, Stanislav Kozubek, Evgeny Krasavin, Martin Falk, Lucie Jezkova, Georg Hildenbrand
Radar Clutters from Inhomogeneities of the Ground Level Environment. Experimental Performances and Statistical Model
Telecommunications and Radio Engineering, Vol.59, 2003, issue 1&2
O.G. Lebedev, L. Z. Sakhnovskaya, V. V. Zhirnov
GRAPHICS AND KNOWLEDGE-BASED OPERATOR SUPPORT
ICHMT DIGITAL LIBRARY ONLINE, Vol.0, 1992, issue
Klaus Zinser, Claus Weisang