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Plasma Medicine
SJR: 0.198 SNIP: 0.183 CiteScore™: 0.57

ISSN Imprimer: 1947-5764
ISSN En ligne: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2016015851
pages 1-12

The Use of an Atmospheric Pressure Plasma Jet to Inhibit Common Wound-Related Pathogenic Strains of Bacteria

Emilia M Kulaga
Plasmology4, Inc., Scottsdale, AZ
David J. Jacofsky
The CORE Institute, Phoenix, AZ
Courtney McDonnell
Plasmology4, Inc., Scottsdale, AZ
Marc C. Jacofsky
Plasmology4, Inc., Scottsdale, AZ; The CORE Institute, Phoenix, AZ


The Plaz4 electrosurgical generator produces a nonequilibrium atmospheric pressure plasma jet (APPJ) at 26°C. This APPJ was tested for its antibacterial capabilities on common wound-related pathogens. The inhibition zone (IZ) of bacterial growth and surviving colony-forming units (CFUs) within the IZ were determined for 4 common clinical isolates: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii. Agar was inoculated at a high density with a bacterial culture medium (107 CFUs/mL). The strains' susceptibility to the APPJ was tested at exposure times between 30 and 140 seconds. A positive correlation between plasma exposure time and bacterial growth IZ size, and a negative correlation between exposure time and the number of surviving CFUs inside the IZ, were observed. It was possible to achieve a round IZ, 4 cm in diameter, for all strains after 70 seconds of exposure, with less than 10 CFUs/cm2 surviving within the zone. P. aeruginosa was more resistant to plasma and required a longer exposure to achieve an IZ similar to that of other strains. However, the number of CFUs surviving inside the IZ was smaller for this strain for a majority of test conditions. Intraoperative contamination is a significant cause of perioperative infection. Drug-resistant bacteria are endemic to hospitals and are a significant public health concern. With the increasing risk of infections related to drug-resistant bacteria, it is crucial to look for alternative treatments. Electrosurgical generators are routinely used in surgical cases. Therefore, Plaz4 applied to surgical sites or debrided wounds could result in a clinically meaningful reduction in tissue bioburden.

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