Publication de 6 numéros par an
ISSN Imprimer: 1045-4403
ISSN En ligne: 2162-6502
IF:
1.6
5-Year IF:
2.2
Immediacy Index:
0.3
Eigenfactor:
0.00058
JCI:
0.33
SJR:
0.345
SNIP:
0.46
CiteScore™::
2.5
H-Index:
67
Indexed in
Association of Interleukin 10 (IL-10) Gene with Type 2 Diabetes Mellitus by Single Nucleotide Polymorphism of Its Promotor Region G/A 1082
Get access
RÉSUMÉ
Interleukin 10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in patients with type 2 diabetes mellitus (T2DM). Cytokines such as IL-10 downregulate the production of pro-inflammatory cytokines, which impair proper function of insulin. So any mutation in the IL-10 gene results in increased production of proinflammatory cytokines, which in turn affect insulin action and cause T2DM. In this study, a polymorphism (rs1800896) in the gene (IL-10) and its association with T2DM was determined with the amplification-refractory mutation system with PCR (ARMS-PCR). Study subjects were divided in two groups, control and T2DM. DNA was extracted from blood, and ARMS-PCR was performed by using specific primers for the promoter region. An amplified product was obtained at 258 base pairs (bp). In the control group, heterozygous bands with both alleles (A/G) were present, whereas AA and GG homozygosity was observed in the T2DM group. This polymorphism (rs1800896) in the IL-10 gene is associated with T2DM. Physical measurements were also obtained, and significant differences between the groups were determined with an independent t-test. Significance was based on a p-value level of 0.05 or less, and highly significant was based on a p-value of 0.01 or less at 95% confidence interval.
-
Garcia TS, Rech TH, Leitao CB. Pancreatic size and fat content in diabetes: A systematic review and meta-analysis of imaging studies. PLoS One. 2017;12(7):e0180911.
-
Zhou B, Lu Y, Hajifathalian K, Bentham J, Di Cesare M, Danaei G, Bixby H, Cowan MJ, Ali MK, Taddei C, Lo WC. Worldwide trends in diabetes since 1980: A pooled analysis of 751 population-based studies with 4.4 million participants. The Lancet. 2016 Apr 9;387(10027):1513-30.
-
Shera AS, Rafique G, Khawaja IA, Baqai S, King H. Pakistan national diabetes survey: Prevalence of glucose intolerance and associated factors in Baluchistan province. Diabetes Res Clin Pract. 1999;44(1):49-58.
-
Bai H, Jing D, Guo A, Yin S. Association between interleukin 10 gene polymorphisms and risk of type 2 diabetes mellitus in a Chinese population. J Int Med Res. 2014;42(3):702-10.
-
Barry JC, Shakibakho S, Durrer C, Simtchouk S, Jawanda KK, Cheung ST, Mui AL, Little JP. Hyporesponsiveness to the anti-inflammatory action of interleukin-10 in type 2 diabetes. Sci Rep. 2016 Feb 17;6:21244.
-
Grimberg J, Nawoschik S, Belluscio L, McKee R, Turck A, Eisenberg A. A simple and effient non-organic procedure for the isolation of genomic DNA from blood. Nucleic Acids Res. 1989;17(20):8390.
-
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes. Diabetes Care. 2018;27(5):1047-53.
-
Shera AS, Jawad F, Maqsood A. Prevalence of diabetes in Pakistan. Diabetes Res Clin Pract. 2007;76(2):219-22.
-
Bays HE, Chapman RH, Grandy S. The relationship of body mass index to diabetes mellitus, hypertension and dyslipidaemia: Comparison of data from two national surveys. Int J Clin Pract. 2007;61(5):737-47.
-
Haase CL, Tybjsrg-Hansen A, Nordestgaard BG, Frikke-Schmidt R. HDL cholesterol and risk of type 2 diabetes: A Mendelian randomization study. Diabetes. 2015;64(9):3328-33.
-
de Boer IH, Bangalore S, Benetos A, Davis AM, Michos ED, Muntner P, Rossing P, Zoungas S, Bakris G. Diabetes and hypertension: A position statement by the American Diabetes Association. Diabetes Care. 2017;40(9): 1273-84.
-
Madeshiya AK, Singh S, Dwivedi S, Konwar R, Natu SM, Ghatak A. Association of IL-10 gene, (-1082A>G, -819C>T and -592C>A) polymorphism and its serum.
-
Asgharzadeh Mohammad, Taghinejad Zahra, Asgharzadeh Vahid, Mehramouz Bahareh, Rashedi Jalil, Mahdavipoor Behroz, Pourostadi Mahya, Vegari Ali, Vishkaei Ali Safarzad, Taghizadeh Sepehr, Kafil Hossein Samadi, Polymorphism of the IL-10 gene in Azeri population of Iran, Egyptian Journal of Medical Human Genetics, 23, 1, 2022. Crossref
-
Effects of Low-Volume High-Intensity Interval Training Compared to Moderate-Intensity Continuous Training on Inflammatory Profile in Women with Type 2 Diabetes, Advances in Geriatric Medicine and Research, 2022. Crossref
-
Albogami Sarah, Hassan Aziza, Abdel-Aziem Sekena H., Alotaibi Saqer, Althobaiti Fayez, El-Shehawi Ahmed, Alnefaie Alaa, Alhamed Reem Abdulla, Effects of combination of obesity, diabetes, and hypoxia on inflammatory regulating genes and cytokines in rat pancreatic tissues and serum, PeerJ, 10, 2022. Crossref
-
Graňák Karol, Vnučák Matej, Beliančinová Monika, Kleinová Patrícia, Pytliaková Margaréta, Miklušica Juraj, Dedinská Ivana, Adiponectin/Leptin Ratio as an Index to Determine Metabolic Risk in Patients after Kidney Transplantation, Medicina, 58, 11, 2022. Crossref