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International Journal of Physiology and Pathophysiology

Publication de 4  numéros par an

ISSN Imprimer: 2155-014X

ISSN En ligne: 2155-0158

SJR: 0.116

Effect of Adenosine Diphosphate on Mitochondrial Respiration of the Pancreatic Acinar Cells in situ

Volume 6, Numéro 1, 2015, pp. 23-34
DOI: 10.1615/IntJPhysPathophys.v6.i1.30
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RÉSUMÉ

Effect of adenosine diphosphate (ADP) on mitochondrial respiration of the rat pancreatic acinar cells in situ was studied using the model of digitonin-treated pancreatic acini. It has been shown that succinate or a mixture of pyruvate, glutamate and malate intensified respiration of permeabilized cells. ADP in low concentration (100 mmol / l) had no effect on the rate of oxygen consumption, while at a higher concentration (750 mmol / l), the short-term intensification of respiration was observed when using nominally calcium-free environment. When reducing the amount of Ca2+ in the medium to 100 nmol /l, ADP had no effect on oxygen consumption, but the rate of respiration, stimulated by a mixture of pyruvate, glutamate and malate, increased. The rate of succinate-stimulated respiration did not depend on the Ca2+ content in the medium. The presence of ATP in the medium reduced the stimulating effect of ADP, while increasing duration of the latter. However, effects of ADP were not inhibited by oligomycin at both succinate oxidation and a mixture of pyruvate, glutamate, and malate. It means that an intensification of respiration of permeabilized acinar pancreacytes by adding ADP in the medium, which is observed only at elevated Ca2+ content, is not associated with oxidative phosphorylation. The effect of ADP may be a novel "functional marker" of pathological processes in mitochondria of acinar pancreacytes.

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