Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Imprimer: 2155-014X
ISSN En ligne: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v6.i3.20
pages 185-189

Effect of Enzymatic Treatment Using Proteases on Properties of Persistent Sodium Current in CA1 Pyramidal Neurons of Rat Hippocampus

Oleksiy O. Lunko
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Dmytro S. Isaev
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Oleksandr P. Maximyuk
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Oleg O. Krishtal
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Olena V. Isaeva
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

RÉSUMÉ

We investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (INaP) in hippocampal CA1 pyramidal neurons. Properties of INaP were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase E (from Streptomyces griseus) or protease type XXIII (from Aspergillus oryzae). We have shown that kinetics of activation and density of INaP in neurons isolated with pronase E was unchanged. Enzymatic treatment with protease type XXIII did not alter INaP activation but resulted in a significant decrease in INaP density. Our data indicate that enzymatic treatment with pronase E for neuron isolation is preferable to investigate INaP.