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International Journal of Physiology and Pathophysiology

Publication de 4  numéros par an

ISSN Imprimer: 2155-014X

ISSN En ligne: 2155-0158

SJR: 0.116

Changes in Synaptic Plasticity due to Blockage of N-type Ca2+ Channels in Cultured Hippocampal Neurons

Volume 1, Numéro 1, 2010, pp. 9-16
DOI: 10.1615/IntJPhysPathophys.v1.i1.20
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RÉSUMÉ

However, it is not known whether presynaptic N-type Ca2+ channels contribute to short-term synaptic plasticity (STP) mediated by GABA release at inhibitory synapses of cultured hippocampal neurons. We studied the sensitivity of GABAergic paired pulse depression (PPD) as a common form of STP to selective N-type high-voltage-activated Ca2+ channels blocker omega-conotoxin (ω-CgTx). Evoked inhibitory postsynaptic currents (eIPSCs) were studied using patch-clamp technique in whole-cell configuration in postsynaptic neuron and local extracellular paired pulse stimulation of single presynaptic axon by rectangular pulse with 0.4 ms duration, the impulse interval in pair was 150 ms. ω-CgTx (200 nM; 1 μM) in a dose-dependent manner irreversibly reduced the amplitude of paired eIPSCs by 25−49% and decreased PPD by 11−22% as compared with control. These results confirm that N-type Ca2+ channels are highly involved in inhibitory synaptic transmission and short-term synaptic plasticity in cultured hippocampal neurons.

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