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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i6.80
pages 595-605

Enhanced Mycelial Biomass Production of the Hairy Bracket Mushroom, Trametes hirsuta (Higher Basidiomycetes), by Optimizing Medium Component with Plackett−Burman Design and Response Surface Methodology

Rongling Yang
Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China
Xueming Liu
Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China
Xiangjie Zhao
Sericulture and Agro-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China
Yujuan Xu
Sericulture and Agro-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
Rongxia Ma
Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China

RÉSUMÉ

Statistical analyses based on experimental designs were applied to optimize the medium components for mycelial biomass production by Trametes hirsuta in shake flask cultivation. First, the effects of different carbon resources (glucose, sucrose, lactose, maltose, fructose, soluble starch and potato), nitrogen resources (yeast extract, peptone, (NH4)2SO4, NH4NO3, NH4Cl, peanut powder, soybean powder) and mineral elements (CaCl2, ZnSO4·7H2O, FeSO4·7H2O, MnSO4·H2O, CuSO4·7H2O) on mycelial biomass production were investigated using a univariate design. Second, a Plackett−Burman design was applied to identify the significant variables that principally influenced the mycelial biomass production, and the path of steepest ascent was pursued to approach the regions of optimal value of the significant variables. Subsequently, these significant variables were optimized using the Box−Behnken design of response surface methodology. Ultimately, the optimized medium conditions were composed of sucrose 25.65 g·L−1, MgSO4·7H2O 1.24 g·L−1, and FeSO4·7H2O 3.36 g·L−1, and the yield of mycelial biomass reached 15.45 g·L−1, which represents an approximately 1.6-fold increase above the initial yield.


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