Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.920
pages 459-460

Use of Agro-Industrial Waste for Production of Laccase and Manganese Peroxidase from White-Rot Basidiomycetes

Moti Rebhun
Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905 Israel
Yitzhak Hadar
Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel


Ligninolytic enzymes have significant potential applications in various industries, including pulp and paper, bioremediation, textile, and laundry. Because the biotechnological applications require large amounts of low-cost enzymes, it is essential to search for powerful producers of enzymes as well as for abundant and inexpensive lignocelluloses wastes. Some of these wastes may contain significant concentrations of soluble carbohydrates and inducers of enzyme synthesis, enabling an efficient production of lignocellulolytic enzymes.
Various agroindustrial byproducts have been used for submerged fermentation of Cerrena unicolor (Bull.: Fr.) Murrill and C. maxima (Fr.) Ryvarden for ligninolytic enzyme production. Six agroindustrial byproducts were tested in shake flask trails. Wheat bran appeared to be the best growth substrate for fermentation of C. unicolor, enabling a very high accumulation of laccase (87.450 IU/L on day 7) in culture liquid. Kiwi, as a growth substrate, supported remarkable secretion of manganese peroxidase (2016 IU/L on day 7). Ethanol production wastes also provided a high yield of laccase, whereas other substrates (banana peels, peanut shells, and cotton stalks) appeared to be rather poor growth substrates for laccase and manganese peroxidase production.
Testing of C. maxima as a ligninolytic enzyme producer showed that this fungus is a weaker producer of laccase and peroxidase than C. unicolor. However, the supplementation of wheat bran, ethanol production wastes, and kiwi highly stimulated laccase production, which reached 6247 IU/L, 6141 IU/L, and 5569 IU/L, respectively. In contrast to C. unicolor, the second tested fungus, C. maxima, produced very low titres of manganese peroxidase (9.2−43.7 IU/L) in fermentation of five lignocellulosic substrates, whereas no manganese peroxidase was detected in submerged fermentation of kiwi. The data prove that the composition of lignocellulose substrates appear to determine the type and amount of enzyme produced by the wood-rotting Basidiomycetes.
Subsequently, C. unicolor cultivation was performed in a 10-liter fermentor using ethanol production wastes as a growth substrate. In order to maximize fungus growth and enzyme production, the pH was automatically controlled at 5.5 during the fermentation process. Laccase activity began on the second day and gradually increased, peaking on the 11th day (233.015 IU/L). Manganese peroxidase showed two peaks, on day 6 (107 IU/L) and on day 10 (142 IU/L).
Fermentation of C. unicolor on wheat bran supported lower laccase levels (185.640 IU/L, 12th day) but a much higher manganese peroxidase yield (6.300 IU/L, 11th day).
Similar to the shake flask trial, alcohol production waste supported quicker laccase production. On the 7th day, alcohol production waste and wheat bran reached 81% and 57% of their maximal laccase concentration, respectively.
We plan to elucidate the specific components in the substrates that affect the kinetics and regulate laccase synthesis. This aspect as well as enhancement of laccase synthesis is crucial for large-scale production. Continuation of yield increase will also include supplementation of inducers as well as further concentration of substrates via batch and fed-batch regimes.

Articles with similar content:

Bioconversion of Plant Raw Materials in Value-Added Products by Lentinus edodes (Berk.) Singer and Pleurotus spp.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George G. Songulashvili, Nana Aladashvili, Yitzhak Hadar, Michel Penninckx, Mikheil D. Asatiani, Eka Metreveli, Vladimir I. Elisashvili
Morpho-physiological Diversity between Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidium (W. Curt.:Fr.) P. Karst. and G. carnosum Pat.
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 5
Ibrahim Hadzic, Jelena Vukojević, Mirjana Stajic, Jasmina Ćilerdžić
Lignocellulose and White-Rot Basidiomycetes: Some Strategies for Their Potential Utilization
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George Kvesitadze, Vladimir I. Elisashvili
Effect of Nutrient Medium Composition on Laccase and Manganese Peroxidase Activity in Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 2
Yitzhak Hadar, Natia V. Kenkebashvili, Vladimir I. Elisashvili
The Growing Effect of Vineyard and Winery Wastes on the Production of Mycelia and Fruit Bodies of Edible and Medicinal Fungi
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George Dicu, Alexandru Teodorescu, Marian Petre