Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.680
427 pages

Cytotoxic Activities of Ergosta-4, 6, 8 (14), 22-tetraen-3-one from the Sclerotia of Grifola umbellata (Pers.) Pilát

Wi Young Lee
Division of Biotechnology, Korea Forest Research Institute, Suwon 441-350, S. Korea
Hak Ju Lee
Div. Wood Chemistry & Microbiology, Korea Forest Research Institute, Seoul 130-712, S. Korea
Youngki Park
Division of Biotechnology, Korea Forest Research Institute, Suwon 441-350, S. Korea
Jin Kwon Ahn
Division of Biotechnology, Korea Forest Research Institute, Suwon 441-350, S. Korea
Kang Hyeon Ka
Div. Wood Chemistry & Microbiology, Korea Forest Research Institute, Seoul 130-712, S. Korea

RÉSUMÉ

Grifola umbellata (also known as Polyporus umbellatus) is a mushroom that is used as a diuretic in Chinese medicine. Herein, we report on the isolation and identification of a cytotoxic compound from the sclerotia of G. umbellata.
For the isolation of the compound, a hexane soluble fraction of the sclerotia of G. umbellata was subjected to column chromatography on silica gel and/or Sephadex LH-20 column eluted with organic solvents. The structure of the isolated compound was elucidated using IR, MS, 1H-, and 13C-NMR spectra, and the structure of the compound was determined as ergosta-4, 6, 8 (14), 22-tetraen-3-one (erogone).
Cytotoxic activities of erogone compared to human cancer cell lines, HT-29 (colon cancer), HeLa 229 (cervix cancer), Hep3B (liver cancer), and AGS (stomach cancer) were compared using the XTT assay kit. Ergone inhibited all cell lines as the dose was increased. In the case of Hep3B and HT-29 cell lines, maximal cytotoxic activities of erogone were achieved at the concentrations of 10 and 15 μ/mL, respectively. However, the cytotoxic activities of erogone compared to HeLa 229 and AGS were much weaker than those of Hep3B and HT-29 cell lines. Values of 50% inhibitory concentrations (IC50) of erogone against Hep3B, HT-29, HeLa 229, and AGS were 5, 7.2, 26.3, and 22 μ/mL, respectively.
Cytotoxic activities of erogone against various tumor cell lines were evaluated in this study for the first time. Therefore, other mushrooms were also examined for the presence of the compound. In the present study, we measured the content of erogone in eight mushrooms—Grifola umbellata, Lentinus edodes (Berk.) Singer, Ganoderma applanatum (Pers.:Wallr.) Pat., Tricholoma matsutake (S.Ito et S.Imai) Singer, Sarcodon aspratus (Berk.) S.Ito, Ramaria botrytis (Pers.) Ricken, Pleurotus eryngii (DC.:Fr.) Quél., and Sparassis crispa (Wulf.)Fr.—and four mycelia—Grifola umbellata, Lentinus edodes, Ganoderma applanatum, and Tricholoma matsutake—using HPLC. The contents of erogone in mushroom and mycelium were in the range of 4.8−29.0 μ/g and 15.5−38.0 μ/g, respectively. Among mushrooms and mycelia tested, the mycelia of Grifola umbellata and Tricholoma matsutake had the highest amounts of erogone (38.0 μ/g).


Articles with similar content:

Selective Cytotoxic Activity of Se-Methyl-Seleno-L-Cysteine– and Se-Polysaccharide–Containing Extracts from Shiitake Medicinal Mushroom, Lentinus edodes (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 8
Maciej Dawidowski, Iwonna Rahden-Staroń, Marzenna Klimaszewska, Jadwiga Turło, Agnieszka Szczepańska, Dagmara Kurpios-Piec, Emilia Orzechowska, Piotr Podsadni, Emilia Grosicka-Maciag, Sandra Górska
Anti-inflammatory Activity of Mycelial Extracts from Medicinal Mushrooms
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 4
Hong-Yu Xu, Zhenming Lu, Yan Geng, Zheng-Hong Xu, Jin-Song Shi, Shuiling Zhu
Contents of Some Metabolites in the Peel and Flesh of the Medicinal Mushroom Wolfiporia cocos (F.A. Wolf) Ryvarden et Gilb. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 1
Yanli Zhao, Tao Li, Ji Zhang, Honggao Liu, Yuanzhong Wang
Isolation and Purification of Polysaccharides with Anti-tumor Activity from Pholiota adiposa (Batsch) P. Kumm. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 3
Hexiang Wang, Qingxiu Hu, Tzi Bun Ng
Antioxidant Properties of Novel Solid-State Fermented Culinary-Medicinal Mushroom and Fungi Products
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 3
Yu-Ling Lee, Tsai-Ping Wu, Hui-Ching Kuo, Shao-Yu Jian, Wan-Lih Huang, Chih-Hung Liang