Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.280
pages 374-375

Nutritional and Medicinal Potential of Twenty-Three Wild Mushrooms from Northeast Thailand

Nipaporn Armassa
Department of Plant Pathology, Faculty of Agriculture, Khon Kaen University
Sompong Thamsirirak
Department of Biochemistry, Faculty of Science, Khon Kaen University, Thailand
Sureelak Rodtong
School of Microbiology, Institute of Science, Suranaree University of Technology, Thailand
Niwat Sanoamuang
Department of Plant Pathology, Faculty of Agiculture, Khon Kaen University, Thailand


Twenty-three species of wild mushrooms (mostly polypores from genera Trametes, Ganoderma, Schizophyllum, Inonotus, Phellinus, and Lentinus) from Sakon Nakhon, northeast Thailand, were collected, and their pure cultures were kept at the Department of Plant Pathology, Khon Kaen University, for further studies.
In this paper, the nutritional value, potential of exo-polysaccharides production, and ability to produce lectins from all species were investigated. In order to produce mycelial biomass, exo-polysaccharides, and lectins culture was grown in a 250 mL Erlenmeyer flask containing 100 mL of rice bran dextrose malt peptone.
(RbDMP) medium at room temperature on a rotary shaker at 125 rpm for 10−15 days. For nutritional value, the mycelial biomass was analyzed for ash, proteins, and Amino, acids contents. The content of ash determined by incineration of mycelia at 500 ± 10°C for 8 hours was very low (approximately 4.73 ± 0.36% of dry matter) in all species. The protein content of all species analyzed through the macro-Kjeldahl method was varied among species, and the average of crude protein was 22.2 ± 5.5% (range 13.3−26.5) of mycelium dry weight. Essential amino acids were determined by using modified techniques (Wiedmeier, Porterfield, and Hendrich, 1982) after hydrolysis of freeze-dried samples with 6 N HC1 for 24 hours at 110°C and analyzed through Shimadzu High Performance Liquid Chromatography. The amino acid patterns were shown clearly, and all amino acids contained in all species were calculated. The amount of the essential amino acids accounted for at least 30.77%, by average, of total amino acids content. Tryptophan could not be detected by the method mentioned above. Essential branch chain amino acids such as isoleucine, leucine, and valine were high, which were useful in treatments involving muscle, mental, and emotional upsets as well as for insommia and nervousness. The amount of exo-polysaccharides was obtained by mixed culture filtrate with 4 vol. of 100% ethanol, stirred vigorously, and kept overnight at 4°C, and the precipitated exo-polysaccharides were air dried and weighted.
Exopolysaccharide production varied considerably among species ranging from 0.33 to 6.59 mg/L with an average of 2.91 ± 0.19 mg/L. Lectin extraction was performed in crude extract by using 5 times (w/v), 10 mM phosphate buffer saline (PBS contained 0.02 M sodium bisulphite, pH 7.2), stirred for 2 hours at 4°C, filtrated and centrifuged for 15 minutes at 12,000 rpm. A hemagglutination test of a serial two-fold dilution of the crude extract and cultured broth was investigated by using Microtiter U-plates with 2% suspension of red blood cells of animals (six kinds) in PBS pH 7.2. Hemagglutination titers of lectins were positively detected at 1:1 (majority) up to 1:16 (1 species) in mycelium, cultured broth, or both in most of the species investigated. All measurements clearly indicated the potential of those wild polypore mushrooms from northeast Thailand as good sources of alternative nutritional food and potential uses as alternative medicine to promote good health and to enhance the body's adaptive capabilities.

Articles with similar content:

Submerged Cultivation of Mycelium with High Ergothioneine Content from the Culinary-Medicinal King Oyster Mushroom Pleurotus eryngii (Higher Basidiomycetes) and its Composition
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Shin-Yi Lin, Jeng-Leun Mau, Ling-Yi Huang, Kung-Jui Ho, Chih-Hung Liang
The Growing Effect of Vineyard and Winery Wastes on the Production of Mycelia and Fruit Bodies of Edible and Medicinal Fungi
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George Dicu, Alexandru Teodorescu, Marian Petre
HS/GC-MS Analyzed Chemical Composition of the Aroma of Fruiting Bodies of Two Species of Genus Lentinus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 5
Ángel Trigos, Remedios Mendoza, Karina Valdez, Gerardo Mata
Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 4
George G. Songulashvili, Yitzhak Hadar, Vladimir I. Elisashvili, Eviatar D. Nevo
Determination of the Biological Efficiency and Antioxidant Potential of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Cultivated Using Different Agro-Wastes in Malaysia
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 1
Ibrahim Alzorqi, Yasmeen Siddiqui, Sivakumar Manickam, Surya Sudheer, Asgar Ali, Poh Guat Cheng