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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v3.i1.70
7 pages

Regulation of Growth and Biosynthetic Activity of the Medicinal Jelly Mushroom Tremella mesenterica (Retz.: Fr.) Pure Culture

Sergey V. Reshetnikov
N. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, 2 Tereshchenkivskaya Str., Kiev 252001, Ukraine; and Med Myco Ltd., Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel
Ina Duckman
Med Myco Ltd., Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel
Katherina Tsukor
Med Myco Ltd., Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel

RÉSUMÉ

Different yeast-like budding haploid strains of the yellow brain mushroom Tremella mesenterica (Retz.: Fr.) were obtained using the monobasidiosporous culture method from basidioma specimens collected in Israel. Nutritional requirements for biomass growth and extracellular polysaccharide production were investigated. A two-stage technology for acidic glucuronoxylomannan production was developed. On a first stage inoculum culture medium was balanced for biomass accumulation, and maximal polysaccharide production was obtained on a fermentation medium under conditions of nitrogen limitation. A crude product obtained by alcohol precipitation of culture broth contains 40% of acidic glucuronoxylomannan, 5% of neutral glucuronoxylomannan, 30% of cell biomass, and also includes low molecular weight substances, free amino acids, and mineral elements.


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