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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v9.i2.30
pages 123-138

Immunomodulatory and Antitumor Activities of Water-Soluble Proteoglycan Isolated from the Fruiting Bodies of Culinary-Medicinal Oyster Mushroom Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae)

Saurabh Shah
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Dipanjan Ghosh
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Sanjaya Kumar Mallick
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Itisam Sarangi
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Sujit Kumar Bhutia
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Indranil Banerje
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Swatilekha Maiti
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Tapas K Maiti
Department of Biotechnology, Indian Institute of Technology Kharagpur, India

RÉSUMÉ

Pleurotus ostreatus, also known as oyster mushroom, is studied for its immunomodulatory and anticancer properties. The present study aimed to find a bioactive proteoglycan from a fruiting body of P. ostreatus that could be used as a biological response modifier for immunomodulatory and anticancer effects with the fewest side effects. A water-soluble fraction of alcohol-precipitated fruiting body extract was purified through anion and cation exchange chromatography, followed by gel filtration (Sephadex G-100) chromatography. The purified proteoglycan fraction was represented by the polysaccharide-to-protein ratio of 3.46. The monosaccharide composition analysis of the proteoglycan showed the presence of galactose, mannose, and glucose in the ratio of 12:11:10. The fraction was examined for its immunomodulatory and anticancer effects in a mouse Sarcoma-180 model. The purified proteoglycan stimulated the growth of thymocytes and splenocytes and induced interleukin-4 and interferon gamma in vitro. It also activated macrophages to produce nitric oxide, super oxide anion, and hydrogen peroxide. The growth inhibition of Sarcoma-180 cells both in vivo and in vitro by this proteoglycan fraction was significant and arrested cell cycle in G0/G1 phase.


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