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International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020033687
pages 197-209

Antitumor Effects of Extract of the Oak Bracket Medicinal Mushroom, Phellinus baumii (Agaricomycetes), on Human Melanoma Cells A375 In Vitro and In Vivo

Yue Yang
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P.R. China
Lan Zhang
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P.R. China
Qian Chen
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P.R. China
Wei-Li Lu
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P.R. China
Ning Li
Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei, P. R. China

RÉSUMÉ

Melanoma is among the most aggressive and treatment-resistant human cancers. Phellinus baumii, a famous medicinal mushroom, has been used to treat different diseases, including cancer, in China and other east Asian countries. The purpose of this research was to explore its anticancer effects against melanoma, and the mechanisms that might be involved. CCK-8 assay exhibited that extracts of Ph. baumii (EPB) strongly inhibited cell viability of A375 melanoma cancer cell. Typical morphological changes of cell apoptosis were observed in EPB-treated A375 cells in Hoechst staining assay. Flow cytometry analysis indicated that EPB significantly induced A375 cells apoptosis and the cell cycle was disrupted in S phase. EPB increased the expression of Bax, and decreased Bcl-2 in A375 cells. EPB remarkably caused mitochondrial membrane potential collapse and induced a mitochondrial-dependent apoptosis in A375 cells evidenced by caspase-3 activation, followed by PARP cleavage. More importantly, EPB has shown a strong inhibitory effect on the migration and aggression of the A375 cells through the healing of the wound and transwell assay. In vivo, EPB was also found to strongly inhibit the growth of tumors in BALB/c nude mice. Our results indicated that Ph. baumii might be a natural therapeutic product for aggressive melanoma because it could induce apoptosis and inhibit metastasis in A375 cells.

RÉFÉRENCES

  1. Shain AH, Bastian BC. From melanocytes to melanomas. Nat Rev Cancer. 2016;16:345-58.

  2. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017. CA Cancer J Clin. 2017;67:7-30.

  3. Sun W, Schuchter LM. Metastatic melanoma. Curr Treat Options Oncol. 2001;2(3):193-202.

  4. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBO-CAN 2008. Int J Cancer. 2010;127:2893-917.

  5. Albuquerque KRS, Pacheco NM, Del Rosario Loyo Casao T, de Melo F, Novaes RD, Goncalves RV. Applicability of plant extracts in preclinical studies of melanoma: a systematic review. Mediators Inflamm. 2018;2018:6797924.

  6. Lebeque Y, Morris HJ, Beltran Y, Llaurado G, Gaime-Perraud I, Meneses M, Moukha S, Bermudez RC, Garcia N. Proximal composition, nutraceutical properties, and acute toxicity study of culinary-medicinal oyster mushroom powder, Pleurotus ostreatus (Agaricomycetes). Int J Med Mushrooms. 2018;20:1185-95.

  7. Venturella G, Saporita P, Gargano ML. The potential role of medicinal mushrooms in the prevention and treatment of gynecological cancers: a review. Int J Med Mushrooms. 2019;21:225-35.

  8. Gupta G, Pathak S, Dahiya R, Awasthi R, Mishra A, Sharma RK, Agrawal M, Dua K. Aqueous extract of wood ear mushroom, Aauricularia polytricha (Agaricomycetes), demonstrated antiepileptic activity against seizure induced by maximal electro-shock and isoniazid in experimental animals. Int J Med Mushrooms. 2019;21:29-35.

  9. Liu Y, Zheng D, Wang D, Su L, Wang Q, Li Y. Immunomodulatory activities of polysaccharides from white button mushroom, Agaricus bisporus (Agaricomycetes), fruiting bodies and cultured mycelia in healthy and immunosuppressed mice. Int J Med Mushrooms. 2019;21:13-27.

  10. Han JG, Hyun MW, Kim CS, Jo JW, Cho JH, Lee KH, Kong WS, Han SK, Oh J, Sung GH. Species identity of Phellinus linteus (sanghuang) extensively used as a medicinal mushroom in Korea. J Microbiol. 2016;54:290-5.

  11. Hwang BS, Lee IK, Choi HJ, Yun BS. Anti-influenza activities of polyphenols from the medicinal mushroom Phellinus baumii. Bioorg Med Chem Lett. 2015;25:3256-60.

  12. Liu MM, Zeng P, Li XT, Shi LG. Antitumor and immunomodulation activities of polysaccharide from Phellinus baumii. Int J Biol Macromol. 2016;91:1199-205.

  13. Zhang H, Shao Q, Wang W, Zhang J, Zhang Z, Liu Y, Yang Y. Characterization of compounds with tumor-cell proliferation inhibition activity from mushroom (Phellinus baumii) mycelia produced by solid-state fermentation. Molecules. 2017;22(5):698.

  14. Kim JE, Takanche JS, Yun BS, Yi HK. Anti-inflammatory character of Phelligridin D modulates periodontal regeneration in lipopolysaccharide-induced human periodontal ligament cells. J Periodontal Res. 2018;53:816-24.

  15. Lee S, Lee D, Jang T, Kang K, Nam J-W, Lee H-J, Kim K. Anti-Inflammatory phenolic metabolites from the edible fungus Phellinus baumii in LPS-stimulated RAW264.7 cells. Molecules. 2017;22:1583.

  16. Yayeh T, Oh WJ, Park SC, Kim TH, Cho JY, Park HJ, Lee IK, Kim SK, Hong SB, Yun BS, Rhee MH. Phellinus baumii ethyl acetate extract inhibits lipopolysaccharide-induced iNOS, COX-2, and proinflammatory cytokine expression in RAW264.7 cells. J Nat Med. 2012;66:49-54.

  17. Wang YY, Ma H, Yan JK, Wang KD, Yang Y, Wang WH, Zhang HN. Three-phase partitioning system with dimethyl carbonate as organic phase for partitioning of exopolysaccharides from Phellinus baumii. Int J Biol Macromol. 2019;131:941-8.

  18. Jin QL, Zhang ZF, Lv GY, Cai WM, Cheng JW, Wang JG, Fan LF. Antioxidant and DNA damage protecting potentials of polysaccharide extracted from Phellinus baumii using a delignification method. Carbohydr Polym. 2016;152:575-82.

  19. Lee IK, Han MS, Lee MS, Kim YS, Yun BS. Styrylpyrones from the medicinal fungus Phellinus baumii and their antioxidant properties. Bioorg Med Chem Lett. 2010;20:5459-61.

  20. Hong SB, Rhee MH, Yun BS, Lim YH, Song HG, Shin KS. Synergistic anti-bacterial effects of Phellinus baumii ethyl acetate extracts and P-lactam antimicrobial agents against methicillin-resistant staphylococcus aureus. Ann Lab Med. 2016;36:111-16.

  21. Noh JR, Lee IK, Kim YH, Gang GT, Hwang JH, Ly SY, Yun BS, Lee CH. Antiatherogenic effect of antioxidant polyphenols from Phellinus baumii in apolipoprotein E-deficient mice. Ann Nutr Metab. 2011;59:145-53.

  22. Wang WH, Wu FH, Yang Y, Wu N, Zhang JS, Feng N, Tang CH. Hypoglycemic effect of ethanol and ethyl acetate extract of Phellinus baumii fruiting body in streptozotocin-induced diabetic mice. Evid Based Complement Alternat Med. 2015;2015:1-7.

  23. Hwang HJ, Kim SW, Lim JM, Joo JH, Kim HO, Kim HM, Yun JW. Hypoglycemic effect of crude exopolysaccharides produced by a medicinal mushroom Phellinus baumii in streptozotocin-induced diabetic rats. Life Sci. 2005;76:3069-80.

  24. Shon YH, Nam KS. Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. J Ethnopharmacol. 2001;77(1):103-9.

  25. Song TY, Lin HC, Yang NC, Hu ML. Antiproliferative and antimetastatic effects of the ethanolic extract of Phellinus igniarius (Linnearus: Fries) Quelet. J Ethnopharmacol. 2008;115:50-6.

  26. Yang Q, Hu XG, Wan Q, Wang WQ. [Study on anti-tumor effect of medicinal fungi Phellinus igniarius extracts]. Zhongguo Zhong Yao Za Zhi. 2006;31(20):1713-5. (in Chinese).

  27. Tschaplinski TJ, Plett JM, Engle NL, Deveau A, Cushman KC, Martin MZ, Doktycz MJ, Tuskan GA, Brun A, Kohler A, Martin F. Populus trichocarpa and Populus deltoides exhibit different metabolomic responses to colonization by the symbiotic fungus Laccaria bicolor. Mol Plant Microbe Interact. 2014;27:546-56.

  28. Wang FF, Liu F, Shi C, Ma W, Wang KJ, Li N. Cytotoxic activities of fractions of the willow bracket medicinal mushroom, Phellinus igniarius (Agaricomycetes), and the induction of cell cycle arrest and apoptosis in MGC-803 cells. Int J Med Mushrooms. 2017;19:561-70.

  29. Chen H, Tian T, Miao H, Zhao YY. Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus: a review. Fitoterapia. 2016;113:6-26.

  30. Ikekawa T, Nakanishi M, Uehara N, Chihara G, Fukuoka F. Antitumor action of some Basidiomycetes, especially Phellinus linteus. Gan. 1968;59:155-7.

  31. Zhang WB, Wang JG, Li ZK, Yang LQ, Qin J, Xiang ZH, Cui HJ. [Progress of studies on medicinal fungus Phellinus]. Zhongguo Zhong Yao Za Zhi. 2014;39:2838-45. (in Chinese).

  32. Ziegler U, Groscurth P. Morphological features of cell death. News Physiol Sci. 2004;19:124-8.

  33. Hacker G. The morphology of apoptosis. Cell Tissue Res. 2000;301(1):5-17.

  34. van Engeland M, Nieland LJ, Ramaekers FC, Schutte B, Reutelingsperger CP. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry. 1998;31:1-9.

  35. Elledge SJ. Cell cycle checkpoints: preventing an identity crisis. Science. 1996;274(5293):1664-72.

  36. Sherr CJ. Cancer cell cycles. Science. 1996;274(5293):1672-7.

  37. Pan Z, Qu C, Chen Y, Chen X, Liu X, Hao W, Xu W, Ye L, Lu P, Li D, Zheng Q. Bufotalin induces cell cycle arrest and cell apoptosis in human malignant melanoma A375 cells. Oncol Rep. 2019;41:2409-17.

  38. Al-Qathama A, Gibbons S, Prieto JM. Differential modulation of Bax/Bcl-2 ratio and onset of caspase-3/7 activation induced by derivatives of Justicidin B in human melanoma cells A375. Oncotarget. 2017;8:95999-96012.

  39. Costa MA, Pellerito L, Izzo V, Fiore T, Pellerito C, Melis M, Musmeci MT, Barbieri G. Diorganotin (IV) and triorganotin (IV) complexes of meso-tetra (4-sulfonatophenyl) porphine induce apoptosis in A375 human melanoma cells. Cancer Lett. 2006;238:284-94.

  40. Kalkavan H, Green DR. MOMP, cell suicide as a BCL-2 family business. Cell Death Differ. 2018;25:46-55.

  41. Zou H, Li Y, Liu X, Wang X. An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem. 1999;274:11549-56.

  42. Boulares AH, Zoltoski AJ, Sherif ZA, Yakovlev A, Smulson ME. Roles of DNA fragmentation factor and poly (ADP-ribose) polymerase-1 in sensitization of fibroblasts to tumor necrosis factor-induced apoptosis. Biochem Biophys Res Commun. 2002;290:796-801.

  43. Hsin MC, Hsieh YH, Wang PH, Ko JL, Hsin IL, Yang SF. Hispolon suppresses metastasis via autophagic degradation of cathepsin S in cervical cancer cells. Cell Death Dis. 2017;8:e3089.


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