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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v17.i12.20
pages 1127-1136

The Shaggy Ink Cap Medicinal Mushroom, Coprinus comatus (Higher Basidiomycetes) Extract Induces Apoptosis in Ovarian Cancer Cells via Extrinsic and Intrinsic Apoptotic Pathways

Amal Rouhana-Toubi
Department of Evolutionary and Environmental Biology and Institute of Evolution, University of Haifa, Mount Carmel, Israel; Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel
Solomon P. Wasser
International Centre for Biotechnology and Biodiversity of Fungi Institute of Evolution and Faculty of Natural Sciences University of Haifa, Mt. Carmel, Haifa 31905, Israel
Fuad Fares
Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Mount Carmel, Israel; Department of Molecular Genetics, Carmel Medical Center, Mount Carmel, Haifa, Israel

RÉSUMÉ

In a previous study, ethyl acetate extract of Coprinus comatus was found to reduce viability of human ovarian cancer cells. The objective of the current research was to clarify the mechanism of action of this extract. Ovarian cancer cells (ES-2) were subjected to ethyl acetate extract of C. comatus for different concentrations or exposure times. Cell cycle analysis and annexin V staining were performed using an automated flow cytometer. DNA fragmentation was detected using the TUNEL assay. Western blot analysis was performed for the assessment of activation of caspases -3, -8, and -9. Results revealed that treatment of ES-2 cells with ethyl acetate extract of C. comatus (100 μg/ml medium), for 48 h or for 72 h resulted in an increased number of cells at the sub-G1 phase of the cell cycle. These treatments also resulted in an increased number of apoptotic cells (positively stained by annexin and positively labeled by TUNEL), in comparison to the control. Reduced levels of procaspases -3, -8, and-9 were also detected in treated cells. In conclusion, ethyl acetate extract of C. comatus induces apoptosis in ovarian cancer cells (ES-2), via both extrinsic and intrinsic pathways. Meanwhile, more investigations are needed to demonstrate weather the apoptotic effect on ovarian cancer cells is accomplished by one active compound, or combined activities of different compounds that exist in the extract.


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