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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v4.i1.60
9 pages

Relationship Between Tyrosinase, Laccase, Peroxidase, and Superoxide Dismutase Activities During the Development of Pleurotus cystidiosus O. K. Miller (Basidiomycota)

Elzbieta Malarczyk
Department of Biochemistry, M. Curie-Sktodowska University, PI. M. Curie-Sktodowskiej 3, Lublin, PoJand
Anna Widenska
Department of Biochemistry, M. Curie-Sktodowska University, PI. M. Curie-Sktodowskiej 3, Lublin, PoJand

RÉSUMÉ

Tyrosinase, laccase, peroxidase. and superoxide dismutase (SOD) activities were investigated in the mycelium and fruiting bodies in various development stages of the fruiting cultures of Pleurotus cystidiosus O. K. Miller grown on a solid sawdust-bran medium. It was found that tyrosinase was present in all mycelium stages and structures but had the highest activity in the mycelium producing melanin-rich coremium type conidia. Peroxidase and SOD activities occurred mainly in fruiting bodies, while laccase was active only in the mycelium. The use of selective inhibitors allowed for tyrosinase and SOD activities differentiation in spectrophotometric assays. Polyacrylamide gel electrophoresis (PAGE) enabled the visualization of active enzymatic protein isoforms. Spectrophotometric assays and PAGE demonstrated the presence of low molecular weight substances showing antioxidant activity in the mycelium.


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