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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018025808
pages 311-320

Characterization and Antioxidant Activity of an Exopolysaccharide Produced by Rigidoporus microporus (Agaricomycetes)

Xuewei Jia
College of Food and Biological Engineering, Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China; Collaborative Innovation Center of Food Production and Safety, Zhengzhou, Henan, China
Lili Qu
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, China
Ran Panpan
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, China
Shuai Liu
College of Life Sciences, Henan Normal University, Henan, China
Yan Wu
Technical Center of China Tobacco Guangxi Industrial Co. Ltd., Nanning, Guanxi, China
Chunping Xu
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, China; Collaborative Innovation Center of Food Production and Safety, Zhengzhou, Henan, China

RÉSUMÉ

In this investigation we developed an optimal fermentation medium to produce an exopolysaccharide (EPS) with antioxidant activity from Rigidoporus microporus. Lactose and tryptone were chosen as the optimal carbon and nitrogen sources, respectively, for EPS production, with a 6-day cultivation cycle. After removing proteins through the use of the Sevag method, one EPS fraction was purified from the culture filtrates by gel filtration chromatography on a Sepharose CL-6B column. The preliminary chemical structure of the EPS fraction was determined by Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance. The results indicated that the main structure of the EPS consisted of β-glucopyranose and mannopyranose. Furthermore, conformational parameters such as molecular weight (Mw), intrinsic viscosity ([η]), mean-square radii of gyration (Rg), and hydrodynamic radius (Rh) of the EPS were characterized using a size exclusion chromatography-multiangle light scattering-refractive index viscometry method. It showed that the EPS was a kind of water-soluble polysaccharide with a moderate molecular weight (34.1 × 104) and a flexible, linear random coil chain structure. The antioxidant activity tests suggested that the EPS has great potential application as a natural antioxidant material in foods and medicines.


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