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International Journal of Medicinal Mushrooms

Publication de 12  numéros par an

ISSN Imprimer: 1521-9437

ISSN En ligne: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Development of an Expression Plasmid and its Use in Genetic Manipulation of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes)

Volume 16, Numéro 2, 2014, pp. 161-168
DOI: 10.1615/IntJMedMushr.v16.i2.60
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RÉSUMÉ

We report the construction of a plasmid, pJW-EXP, designed for the expression of homologous and heterologous genes in Ganoderma lucidum. pJW-EXP was generated from the plasmid pMD19-T by inserting the G. lucidum glyceraldehyde-3-phosphate dehydrogenase gene promoter, the G. lucidum iron-sulfur protein subunit of succinate dehydrogenase gene terminator and the homologous carboxin-resistance gene as selection marker. This expression plasmid can be efficiently transformed into Ganoderma through polyethylene glycol-mediated protoplast transformation. Southern blot analysis showed that most of the integrated DNA appeared as multiple copies in the genome. The applicability of the constructed plasmid was tested by expression of the truncated G. lucidum 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene that encodes the catalytic domain of HMGR. Overexpression of the truncated HMGR gene, which is a key gene in the biosynthetic pathway of the antitumor compounds, ganoderic acids, increased the transcription of the HMGR gene and enhanced ganoderic acid accumulation. pJW-EXP can serve as a useful tool in the genetic improvement and metabolic engineering of Ganoderma.

CITÉ PAR
  1. Zhou Jiang-Sheng, Ji Sen-Lin, Ren Meng-Fei, He Yi-Long, Jing Xiao-Ran, Xu Jun-Wei, Enhanced accumulation of individual ganoderic acids in a submerged culture of Ganoderma lucidum by the overexpression of squalene synthase gene, Biochemical Engineering Journal, 90, 2014. Crossref

  2. Xu Jun-Wei, Ji Sen-Lin, Li Huan-Jun, Zhou Jiang-Sheng, Duan Yan-Qing, Dang Li-Zhi, Mo Ming-He, Increased polysaccharide production and biosynthetic gene expressions in a submerged culture of Ganoderma lucidum by the overexpression of the homologous α-phosphoglucomutase gene, Bioprocess and Biosystems Engineering, 38, 2, 2015. Crossref

  3. Li Huan-Jun, Zhang De-Huai, Yue Tong-Hui, Jiang Lu-Xi, Yu Xuya, Zhao Peng, Li Tao, Xu Jun-Wei, Improved polysaccharide production in a submerged culture of Ganoderma lucidum by the heterologous expression of Vitreoscilla hemoglobin gene, Journal of Biotechnology, 217, 2016. Crossref

  4. Kilaru S., Schuster M., Latz M., Das Gupta S., Steinberg N., Fones H., Gurr S.J., Talbot N.J., Steinberg G., A gene locus for targeted ectopic gene integration in Zymoseptoria tritici, Fungal Genetics and Biology, 79, 2015. Crossref

  5. Xu Jun-Wei, Zhong Jian-Jiang, Genetic engineering ofGanoderma lucidumfor the efficient production of ganoderic acids, Bioengineered, 6, 6, 2015. Crossref

  6. Li Huan-Jun, Zhang De-Huai, Han Li-Liang, Yu Xuya, Zhao Peng, Li Tao, Zhong Jian-Jiang, Xu Jun-Wei, Further improvement in ganoderic acid production in static liquid culture of Ganoderma lucidum by integrating nitrogen limitation and calcium ion addition, Bioprocess and Biosystems Engineering, 39, 1, 2016. Crossref

  7. Li Huan-Jun, He Yi-Long, Zhang De-Huai, Yue Tong-Hui, Jiang Lu-Xi, Li Na, Xu Jun-Wei, Enhancement of ganoderic acid production by constitutively expressing Vitreoscilla hemoglobin gene in Ganoderma lucidum, Journal of Biotechnology, 227, 2016. Crossref

  8. Zhang De-Huai, Li Na, Yu Xuya, Zhao Peng, Li Tao, Xu Jun-Wei, Overexpression of the homologous lanosterol synthase gene in ganoderic acid biosynthesis in Ganoderma lingzhi, Phytochemistry, 134, 2017. Crossref

  9. Qin Hao, Xiao Han, Zou Gen, Zhou Zhihua, Zhong Jian-Jiang, CRISPR-Cas9 assisted gene disruption in the higher fungus Ganoderma species, Process Biochemistry, 56, 2017. Crossref

  10. Zhou Xuan-Wei, Cultivation ofGanoderma lucidum, in Edible and Medicinal Mushrooms, 2017. Crossref

  11. Gu Li, Zheng Yimei, Lian Danhong, Zhong Xin, Liu Xin, Production of triterpenoids from Ganoderma lucidum : Elicitation strategy and signal transduction, Process Biochemistry, 69, 2018. Crossref

  12. Ren Ang, Shi Liang, Zhu Jing, Yu Hanshou, Jiang Ailiang, Zheng Huihua, Zhao Mingwen, Shedding light on the mechanisms underlying the environmental regulation of secondary metabolite ganoderic acid in Ganoderma lucidum using physiological and genetic methods, Fungal Genetics and Biology, 128, 2019. Crossref

  13. Qin Hao, Xu Jun-Wei, Xiao Jian-Hui, Tang Ya-Jie, Xiao Han, Zhong Jian-Jiang, Cell Factories of Higher Fungi for Useful Metabolite Production, in Bioreactor Engineering Research and Industrial Applications I, 155, 2015. Crossref

  14. Fei Yu, Li Na, Zhang De-Huai, Xu Jun-Wei, Increased production of ganoderic acids by overexpression of homologous farnesyl diphosphate synthase and kinetic modeling of ganoderic acid production in Ganoderma lucidum, Microbial Cell Factories, 18, 1, 2019. Crossref

  15. Liu Ke, Sun Bin, You Hao, Tu Jun‐Liang, Yu Xuya, Zhao Peng, Xu Jun‐Wei, Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system , Microbial Biotechnology, 13, 2, 2020. Crossref

  16. Ren Ang, Shi Liang, Zhu Jing, Liu Rui, Zhao Mingwen, Biosynthetic Pathway and Signal Transduction Regulation of Ganoderic Acid Biosynthesis in Lingzhi, in The Lingzhi Mushroom Genome, 2021. Crossref

  17. Xu Jun-Wei, Genetic Transformation System, in The Lingzhi Mushroom Genome, 2021. Crossref

  18. Zhang De-Huai, Jiang Lu-Xi, Li Na, Yu Xuya, Zhao Peng, Li Tao, Xu Jun-Wei, Overexpression of the Squalene Epoxidase Gene Alone and in Combination with the 3-Hydroxy-3-methylglutaryl Coenzyme A Gene Increases Ganoderic Acid Production in Ganoderma lingzhi, Journal of Agricultural and Food Chemistry, 65, 23, 2017. Crossref

  19. Wang Zi-Xu, Li Na, Xu Jun-Wei, Effects of Efficient Expression of Vitreoscilla Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in Ganoderma lucidum, Microorganisms, 9, 8, 2021. Crossref

  20. You Hao, Sun Bin, Li Na, Xu Jun-Wei, Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum, Microbial Cell Factories, 20, 1, 2021. Crossref

  21. Lim Fook-Hwa, Rasid Omar Abd, Idris Abu Seman, As’wad Abdul Wahab Mohd, Vadamalai Ganesan, Parveez Ghulam Kadir Ahmad, Wong Mui-Yun, Enhanced polyethylene glycol (PEG)–mediated protoplast transformation system for the phytopathogenic fungus, Ganoderma boninense, Folia Microbiologica, 66, 4, 2021. Crossref

  22. Zhang Qi, Yuan Chang, Wang Feng, Xu Sha, Li Youran, Shi Guiyang, Ding Zhongyang, Roles of Small Subunits of Laccase (ssPOXA3a/b) in Laccase Production by Pleurotus eryngii var. ferulae, Journal of Agricultural and Food Chemistry, 69, 44, 2021. Crossref

  23. Ahmad Md Faruque, Ahmad Fakhruddin Ali, Khan Mohammed Idreesh, Alsayegh Abdulrahman A., Wahab Shadma, Alam M. Intakhab, Ahmed Faiyaz, Ganoderma lucidum: A potential source to surmount viral infections through β-glucans immunomodulatory and triterpenoids antiviral properties, International Journal of Biological Macromolecules, 187, 2021. Crossref

  24. Ahmad Md Faruque, Wahab Shadma, Ahmad Fakhruddin Ali, Ashraf Syed Amir, Abullais Shahabe Saquib, Saad Hisham H., Ganoderma lucidum: A potential pleiotropic approach of ganoderic acids in health reinforcement and factors influencing their production, Fungal Biology Reviews, 39, 2022. Crossref

  25. Zhang Qi, Zhao Liting, Shen Mengye, Liu Jingyun, Li Youran, Xu Sha, Chen Lei, Shi Guiyang, Ding Zhongyang, Establishment of an Efficient Polyethylene Glycol (PEG)-Mediated Transformation System in Pleurotus eryngii var. ferulae Using Comprehensive Optimization and Multiple Endogenous Promoters, Journal of Fungi, 8, 2, 2022. Crossref

  26. Tu Jun-Liang, Bai Xin-Yuan, Xu Yong-Liang, Li Na, Xu Jun-Wei, Atomi Haruyuki, Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9, Applied and Environmental Microbiology, 87, 23, 2021. Crossref

  27. Zhang Hao, Zhang Xiaoyu, Geng Anli, Expression of a novel manganese peroxidase from Cerrena unicolor BBP6 in Pichia pastoris and its application in dye decolorization and PAH degradation, Biochemical Engineering Journal, 153, 2020. Crossref

  28. Zhao Li-Na, Cao Yu-Biao, Luo Qin, Xu Yong-Liang, Li Na, Wang Cheng-Xiao, Xu Jun-Wei, Overexpression of phosphomannomutase increases the production and bioactivities of Ganoderma exopolysaccharides, Carbohydrate Polymers, 294, 2022. Crossref

  29. Luo Qin, Li Na, Xu Jun-Wei, A methyltransferase LaeA regulates ganoderic acid biosynthesis in Ganoderma lingzhi, Frontiers in Microbiology, 13, 2022. Crossref

  30. Ab Wahab Anis Farhan Fatimi, Ahmad Zairun Madihah , Mohd Daud Khairunnisa Hanisah, Abu Bakar Farah Diba , Bharudin Izwan , Abdul Murad Abdul Munir, Evaluation and Improvement of Protocols for Ganoderma boninense Protoplast Isolation and Regeneration, Malaysian Applied Biology, 51, 5, 2022. Crossref

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