Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i4.70
pages 409-418

Submerged and Solid-State Cultivation of Bioactive Extra- and Intracellular Polysaccharides of Medicinal Mushrooms Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and Grifola frondosa (Dicks.: Fr.) S. F. Gray (Aphyllophoromycetideae)

Jozica Habijanic
National Institute of Chemistry, Ljubljana, Slovenia ; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia
Mirjan Svagelj
Department of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana
Marin BEROVIC
Prof.Dr.Bioche.Eng.
Bojana Boh
Faculty of Natural Sciences and Engineering, University of Ljubljana, Ljubljana, Slovenia
Branka Wraber
Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia

RÉSUMÉ

Original strains of Ganoderma lucidum (MZKI G97) and Grifola frondosa (GF3) isolated from Slovenian forests were cultivated using submerged and solid-state cultivation. In G. lucidum after 14 days of submerged fed-batch cultivation, up to 17.0 g L−1 dry fungal biomass was produced. Extracellular (1.7 g L−1) and intracellular (0.45 g L−1) polysaccharide fractions were isolated, while in 18 days of solid-state cultivation, 5.77 mg/g of extracellular and 1.45 mg/g of intracellular polysaccharide were produced at the end of the cultivation period. In 28 days of submerged cultivation of G. frondosa, 3.65 mg L−1 of extracellular and 1.30 mg L−1 of intracellular polysaccharide were produced. In 38 days of solid-state cultivation, 3.80 mg/g of extracellular and 0.70 mg/g of intracellular polysaccharide were produced. Polysaccharides were further separated by ion-exchange, gel, and affinity chromatography. The isolated polysaccharides were mainly β-D-glucans. Immunostimulatory effects of isolates were tested for induction of cytokine (TNF-α and IFN-γ) synthesis in human peripheral blood mononuclear cells (PBMC) isolated from buffy coat.


Articles with similar content:

Ganoderma lucidum (W.Curt.:Fr.) Lloyd and G. applanatum (Pers.) Pat. (Aphyllophoromycetideae) from Slovenian Habitats: Cultivation, Isolation, and Testing of Active Compounds
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 1
Irena Zore, Branka Wraber, Jozica Habijanic, Damjan Hodzar, Bojana Boh, Marin BEROVIC
The Medicinal Benefits of Lentinan (β-1, 3-D glucan) from Lentinus edodes (Berk.) Singer (Shiitake Mushroom) Through Oral Administration
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 1&2
Mah-Lee Ng, Ann-Teck Yap
Immunomodulating Activities of Cultivated Maitake Medicinal Mushroom Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes) on Peripheral Blood Mononuclear Cells
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 4
Sasa Simcic, Branka Wraber, Andrej Gregori, Mirjan Svagelj, Bojana Boh, Marin BEROVIC
High-Level Production of Exopolysaccharides by a Cosmic Radiation−Induced Mutant M270 of the Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 7
Ai-Rong Song, Chen Zhao, Wen-Xing Liang, Guang-Yuan Wang, Xue-Mei Tian
Role of Two Species of Medicinal Mushrooms from Genus Ganoderma P. Karst. (G. lucidum and G. tsugae; Aphyllophoromycetideae) in Selective Estrogen-Receptor Modulations
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 1
Jun Gong, Jing Qin, Vivien Wing-Yin Luk, Siu Wai Chiu