Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
Indexed in
Apoptotic Effect of Extract from Medicinal Mushroom from Taiwan Taiwanofungus salmoneus (Higher Basidiomycetes) Mycelium Combined with or without Cisplatin on Hepatocellular Carcinoma Cells
RÉSUMÉ
Hepatocellular carcinoma is a cancer of high mortality; therefore, the effective therapy on this cancer is an imperative issue. Recently, anticancer agent combined with natural products has been demonstrated to increase apoptosis of various cancer cells effectively. Accordingly, we investigated the apoptotic effect and possible mechanism of the ethanol extract from Taiwanofungus salmoneus (=Antrodia salmonea) mycelium (TsE) alone or in combination with cisplatin in SK-Hep-1 cells. In this study, the proliferation of SK-Hep-1 cells could be inhibited at various concentrations of TsE for 24 h whereas TsE combined with cisplatin would inhibit the cell proliferation more notably. Moreover, the DNA damage and the interruption of cell cycle of SK-Hep-1 cells would be effectively raised after incubation with TsE combined with cisplatin for 24 h. The apoptosis of cells was dramatically induced, and the expression of caspases 3, 8, and 9, apoptosis-related protein, were significantly upregulated. Therefore, we proposed that the TsE combined with cisplatin inhibited cell proliferation by elevating sub-G1 phase, inducing DNA damage, activating caspases 3, 8, and 9 activities, and triggering cells apoptosis. These results reveal that TsE could be a potential adjuvant chemotherapeutic agent.
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Chang Chia-Ting, Hseu You-Cheng, Thiyagarajan Varadharajan, Huang Hui-Chi, Hsu Li-Sung, Huang Pei-Jane, Liu Jer-Yuh, Liao Jiunn-Wang, Yang Hsin-Ling, Antrodia salmonea induces G2 cell-cycle arrest in human triple-negative breast cancer (MDA-MB-231) cells and suppresses tumor growth in athymic nude mice, Journal of Ethnopharmacology, 196, 2017. Crossref
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Karuppaiya Palaniyandi, Akbar Abdul Khader, Mycotherapy of Antrodia salmonea: A Taiwanese Medicinal Mushroom, in Medicinal Mushrooms, 2019. Crossref
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Lin Chun-Ping, Wu Yao-Chien, Lin Zhi-Wei, Tsai Shu-Yao, Assessment of the thermal and oxidative stability of Antrodia salmonea extract in dripping pills via DSC and TAM air analyses, Journal of Thermal Analysis and Calorimetry, 144, 2, 2021. Crossref
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Nowakowski Patryk, Markiewicz-Żukowska Renata, Bielecka Joanna, Mielcarek Konrad, Grabia Monika, Socha Katarzyna, Treasures from the forest: Evaluation of mushroom extracts as anti-cancer agents, Biomedicine & Pharmacotherapy, 143, 2021. Crossref