Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
IF:
1.2
5-Year IF:
1.4
Immediacy Index:
0.3
Eigenfactor:
0.00066
JCI:
0.34
SJR:
0.274
SNIP:
0.41
CiteScore™::
2.8
H-Index:
37
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Inhibitory Effects of a Polysaccharide Extract from the Chaga Medicinal Mushroom, Inonotus obliquus (Higher Basidiomycetes), on the Proliferation of Human Neurogliocytoma Cells
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RÉSUMÉ
This study aimed to investigate the inhibitory roles of a polysaccharide extract from Inonotus obliquus on U251 human neurogliocytoma cells cultured in vitro. After administering the polysaccharide extract from I. obliquus to U251 cells cultivated in vitro, methyl thiazolyl tetrazoliym assay was performed to measure the inhibitory effects of the extract on tumor cell proliferation. The expression of the apoptosis-related proteins Bcl-2 and caspase-3 were determined by Western blotting. Different concentrations of I. obliquus extract (25, 50, 100, 200, and 500 µg/mL) were added to U251 cells at 24, 48, and 72 hours. Methyl thiazolyl tetrazoliym assay showed that the inhibition ratio increased with increased extract concentration and prolonged treatment duration. The I. obliquus extract sharply decreased the expression of Bcl-2 but dramatically increased the expression of caspase-3. This function was gradually enhanced with increased drug concentration and prolonged treatment duration. The I. obliquus extract can inhibit the proliferation of tumor cells. This inhibition function is closely related to the downregulation of Bcl-2 and the upregulation of caspase-3.
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Liang Zengenni, Guo Yu-Tong, Yi You-Jin, Wang Ren-Cai, Hu Qiu-Long, Xiong Xing-Yao, Ganoderma Lucidum Polysaccharides Target a Fas/Caspase Dependent Pathway to Induce Apoptosis in Human Colon Cancer Cells, Asian Pacific Journal of Cancer Prevention, 15, 9, 2014. Crossref
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Lau Beng Fye, Abdullah Noorlidah, Sclerotium-Forming Mushrooms as an Emerging Source of Medicinals, in Mushroom Biotechnology, 2016. Crossref
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Ghedira K., Goetz P., Chaga: Inonotus obliquus (Hymenochaetaceae), Phytothérapie, 16, 5, 2018. Crossref
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Parfenov A. A., Vyshtakalyuk A. B., Sysoeva M. A., Sysoeva E. V., Latipova A. D., Gumarova L. F., Zobov V. V., Hepatoprotective Effect of Inonotus obliquus Melanins: In Vitro and In Vivo Studies, BioNanoScience, 9, 2, 2019. Crossref
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Patel Seema, Immunomodulatory Aspects of Medicinal Mushrooms, in Medicinal Mushrooms, 2019. Crossref
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Eid Jehane Ibrahim, Das Biswadeep, Molecular insights and cell cycle assessment upon exposure to Chaga (Inonotus obliquus) mushroom polysaccharides in zebrafish (Danio rerio), Scientific Reports, 10, 1, 2020. Crossref
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Badalyan Susanna M., Rapior Sylvie, The Neurotrophic and Neuroprotective Potential of Macrofungi, in Medicinal Herbs and Fungi, 2021. Crossref
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Zhao Yanxia, Zheng Weifa, Deciphering the antitumoral potential of the bioactive metabolites from medicinal mushroom Inonotus obliquus, Journal of Ethnopharmacology, 265, 2021. Crossref
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Razumov E, Safin R, Mukhametzyanov S R, Hučko Boris, Martin Pavlik, Gomba Gordon Karikoga, Bazant Vaclav, Influence of development conditions on qualitative indicators of Fungus Chaga, IOP Conference Series: Materials Science and Engineering, 666, 1, 2019. Crossref
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Li Jiawei, Qu Chao, Li Fangfang, Chen Yifang, Zheng Jinjuan, Xiao Yao, Jin Quanxin, Jin Guihua, Huang Xuezhu, Jin Dan, Inonotus obliquus Polysaccharide Ameliorates Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Cancer in Mice via Activation of the NLRP3 Inflammasome, Frontiers in Pharmacology, 11, 2021. Crossref
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Kothari Damini, Patel Seema, Kim Soo-Ki, Anticancer and other therapeutic relevance of mushroom polysaccharides: A holistic appraisal, Biomedicine & Pharmacotherapy, 105, 2018. Crossref
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Nowakowski Patryk, Markiewicz-Żukowska Renata, Bielecka Joanna, Mielcarek Konrad, Grabia Monika, Socha Katarzyna, Treasures from the forest: Evaluation of mushroom extracts as anti-cancer agents, Biomedicine & Pharmacotherapy, 143, 2021. Crossref