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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v11.i1.90
pages 77-86

Genomic Cloning and Characterization of a FIP-gsi Gene Encoding a Fungal Immunomodulatory Protein from Ganoderma sinense Zhao et al. (Aphyllophoromycetideae)

Xuan-Wei Zhou
School of Agriculture and Biology and Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai, People's Republic of China
Minqi Xie
Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Fan Hong
Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Qi-zhang Li
Plant Biotechnology Research Center, Shanghai Key Laboratory of Agro-biotechnology, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai 200240, P. R. China

RÉSUMÉ

A genomic DNA sequence encoding fungal immunomodulatory protein (FIP), the FIP-gsi gene, was isolated from Ganoderma sinense (Ganodermataceae, Basidiomycetes) using genomic walker technology. Analysis of 1072-bp segments revealed that the gene contained a 501-bp 5'-flanking region, a 333-bp open read frame (ORF), and a 238-bp 3'-flanking region. There is one putative TATA box and one possible CAAT box lieing in the 5'-flanking region. The ORF encodes a 12.4-kDa precursor polypeptide. One intron was found in the 5'-flanking region. The deduced amino acid sequence of FIP-gsi shares high similarity with other FIPs, for example, G. lucidum (FIP-glu), G. japonicum (FIP-gja), G. tsugae (FIP-gts), Flammulina velutipes (FIP-fve), and Volvariella volvacea (FIP-vvo), which were found to be 99%, 86%, 86%, 61%, and 56% identical, respectively. The cloning of the genomic DNA sequence is an important foundation for further study of its structure, expression, and regulatory mechanisms.


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