Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018026571
pages 647-655

Characteristics of Exopolysaccharides from the Citrine Oyster Mushroom, Pleurotus citrinopileatus (Agaricomycetes), Depend on the Nitrogen Source in the Medium

Chiu-Yeh Wu
Department of Culinary Arts, Chung Chou University of Science and Technology, Changhua, Taiwan, Republic of China
Zeng-Chin Liang
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan

RÉSUMÉ

We investigated the effect of peptone and (NH4)2SO4, used as nitrogen sources in a submerged medium, on the molecular weight and carbohydrate compositions of exopolysaccharides (EPSs) produced by the culinary-medicinal mushroom Pleurotus citrinopileatus. When (NH4)2SO4 was used instead of peptone, it yielded more specific EPSs, producing 32.80 mg EPS/g dry mycelial biomass, but small amounts of EPS and mycelial biomass. These nitrogen sources also resulted in the production of different net charged EPSs, according to crude fractionations on a DEAE Sepharose Fast Flow column. Purified EPSs were initially repurified by gel filtration chromatography, and the profiles indicated that a range of EPSs with different molecular weights were collected through the use of this strategy. Analysis of the carbohydrate composition of purified EPSs demonstrated that the main component produced by P. citrinopileatus in a glucose-(NH4)2SO4 medium, with an estimated molecular weight < 10 kDa, was rich in glucose (95.9%), and its structure could consist of glucan. Conversely, this mushroom produced diverse heteropolysaccharides (11.1-42.3% mannose, 6.3-80.3% glucose, 0.3-49.2% N-acetylgalactosamine, and 7.6-26.3% N-acetylglucosamine) in a glucose-peptone submerged medium. By applying various nitrogen sources in submerged medium, these results could be used to improve the possibility of obtaining molecules similar to those found in the fruiting bodies of the same mushroom species grown in conventional culture.


Articles with similar content:

High-Level Production of Exopolysaccharides by a Cosmic Radiation−Induced Mutant M270 of the Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 7
Ai-Rong Song, Chen Zhao, Wen-Xing Liang, Guang-Yuan Wang, Xue-Mei Tian
Production of Basidiomata and Ligninolytic Enzymes by the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Licuri (Syagrus coronata) Wastes in Brazil
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 12
Luciana Porto de Souza Vandenberghe, Aline Simoes da Rocha Bispo, Helio Mitoshi Kamida, Maria Gabriela Bello Koblitz, Aristoteles Goes-Neto, Thais Almeida de Menezes
Production of Biomass, Polysaccharides, and Ganoderic Acid using Non-conventional Carbon Sources under Submerged Culture of the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 2
Diego F. Rojas, Paola A. Zapata, Lucia Atehortua
Comparative Study of Lectin Activity of Higher Basidiomycetes
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 1
Solomon P. Wasser, Nona A. Mikiashvili, Vladimir I. Elisashvili, Eviatar D. Nevo
Antimicrobial Effect of the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) and Its Main Compounds
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 1
Mahdi Vazirian, Azadeh Manayi, Ali Asghari, Nasrin Samadi, Yaghoub Amanzadeh, Seyed Esmaeil Sadat Ebrahimi, Saeed Ali Mousazadeh, Mohammad Reza Asef, Mohammad Ali Faramarzi, Emran Habibi, Hamid Reza Monsef Esfahani, Seyed Aboulfazl Hosseini