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International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018026044
pages 419-429

Nephroprotective and Antioxidant Effects of King Tuber Oyster Medicinal Mushroom, Pleurotus tuber-regium (Agaricomycetes), on Carbon Tetrachloride-Induced Nephrotoxicity in Male Sprague Dawley Rats

Kenneth O. Okolo
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Madonna University, Port Harcourt, Elele Rivers State, Nigeria
Orish Ebere Orisakwe
Toxicology Unit, Faculty of Pharmacy, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria
Iyeopu M. Siminialayi
Department of Pharmacology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria

RÉSUMÉ

Chronic kidney disease is a health burden worldwide but particularly in sub-Saharan Africa, with dwindling resources as a result of poor commodity export, devaluation of currencies, and corruption that had decreased the average family income and substantially increased the number of persons living on less than $1.90/day. Natural products are part of the healthcare delivery system in that part of the world. This study was conducted to evaluate the protective effects of Pleurotus tuber-regium on the kidneys of rats treated with carbon tetrachloride (CCl4) for 13 weeks. Sixty rats were divided into 6 groups, with 10 animals in each group. Group I (control) received olive oil (3 mL/kg) intraperitoneally twice weekly and were given feed and water ad libitum. Group II received CCl4 (3 mL/kg, 30% in olive oil) twice weekly. Groups III, IV, and V received 100, 200, and 500 mg/kg wild edible P. tuber-regium (33.3% in feed) daily, respectively, in addition to 3 mL/kg CCl4 twice weekly. Group VI received 500 mg/kg P. tuber-regium (33.3% in feed) daily. At the end of 13 weeks, the animals were sacrificed and kidney weights recorded. Serum concentrations of creatinine, urea, and fasting blood glucose were assayed. Malondialdehyde, ascorbic acid, α-tocopherol, superoxide dismutase, catalase, total glutathione, and peroxidase were measured in kidney homogenate. The kidneys were also histologically examined. Administration of CCl4 to rats significantly (P < 0.05) increased the absolute and relative kidney weights from 0.93 ± 0.04 and 0.38 ± 0.02 g in the control group to 1.30 ± 0.04 and 0.58 ± 0.02 g in the treated groups (Groups III, IV, and V), respectively. CCl4 administration increased the concentrations of creatinine, urea, fasting blood glucose, and malondialdehyde from 0.53 ± 0.05 mg/dL, 17.0 ± 1.10 mg/dL, 72 ± 2.80 mg/dL, and 1.40 ± 0.32 μmol/L in the control group to 0.84 ± 0.03 mg/dL, 43.0 ± 6.90 mg/dL, 77 ± 2.2 mg/dL, and 14.0 ± 3.5 μmol/L in the treated groups, respectively. The concentrations of ascorbic acid, α-tocopherol, superoxide dismutase, catalase, total glutathione, and peroxidase decreased significantly (P < 0.05) from 0.41 ± 0.02 mg/dL, 5.15 ± 0.21 μg/mL, 8.49 ± 0.38 units/mL, 75.20 ± 4.57 mU/mL, 1.62 ± 0.03 μg/mL, and 9.74 ± 0.40 mU/mL in the control group to 0.24 ± 0.03 mg/dL, 1.80 ± 0.11 μg/mL, 2.78 ± 0.30 units/mL, 31.9 ± 5.87 mU/mL, 0.36 ± 0.04 μg/mL, and 3.84 ± 0.22 mU/mL in the treated groups, respectively. Photomicrographs showed that P. tuber-regium prevented the fibrosis and tubular and Bowman capsule distortions observed in the CCl4-only group. P. tuber-regium is effective in protecting the kidneys against CCl4-induced damage.