Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i6.40
pages 521-533

Immunomodulatory Effects of Extracellular β-Glucan Isolated from the King Oyster Mushroom Pleurotus eryngii (Agaricomycetes) and Its Sulfated Form on Signaling Molecules Involved in Innate Immunity

Yong Hyun Kim
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea; BMI Korea Co. Ltd., Uiwang-si, Gyeonggi-Do, Republic of Korea
Eui-Gil Jung
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Kook-Il Han
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Bharat Bhusan Patnaik
Trident School of Biotech Sciences, Trident Academy of Creative Technology, Chandrasekharpur, Bhubaneswar, Odisha, India
Hyun-Jung Kwon
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Hyoun-Su Lee
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Wan Jong Kim
BMI Korea Co. Ltd., Uiwang-si, Gyeonggi-Do, Republic of Korea
Man-Deuk Han
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea

RÉSUMÉ

The aim of this study was to determine, using murine RAW 264.7 macrophages, the immunomodulatory effect of extracellular β-glucan isolated from Pleurotus eryngii (PEBG) and its sulfated derivative (PEBG-S) on signaling molecules implicated in host innate immunity. β-Glucan was extracted and purified from the mycelial culture using optimal medium concentrations. It was then chemically converted to its sulfated form. Monosaccharide composition of β-glucan was characterized with p-aminobenzoic acid ethyl ester–derivatized sugars through highperformance liquid chromatography analysis. Fourier transform infrared structural analysis showed an S=O bond at 1250 cm-1 and C-S-O binding at 815 cm-1 in PEBG-S. 13C nuclear magnetic resonance analysis showed 1,3-linked α-D-mannopyranosyl and 1,3-β-D-glucopyranosyl in PEBG-S. A concentration-dependent increase of nitric oxide production was noticed in RAW 264.7 cells treated with PEBG-S or PEBG; those treated with PEBG-S showed less cytotoxicity than those treated with PEBG. Cellular levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6 were increased by PEBG and PEBG-S treatment, suggesting that they have immunomodulatory activity. Real-time polymerase chain reaction array revealed that the expression levels of nuclear factor-κB and Toll-like receptor signaling genes in cells were upregulated by PEBG and PEBG-S. Moreover, the expression of the β-glucan receptor dectin-2 was significantly upregulated by PEBG and PEBG-S treatment, reflecting immune activation through the dectin-2-Syk-(CARD9/Bcl-10/MALT1) pathway. Our results suggest that PEBG-S could be used as an effective adjuvant or immune enhancer that can be sustainably produced by recycling the by-product of mycelial culture.


Articles with similar content:

A Polysaccharide from the Culinary-Medicinal Mushroom Pholiota nameko (Agaricomycetes) Inhibits the NF-κB Pathway in Dendritic Cells Through the TLR2 Receptor
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Guoliang Li, Lihua Huai, Yufeng Xu, Haiping Li, Ruixuan Zhu, Fengling Wang, Pei Zhao
The Inhibitory Properties of Ethanol Extracts of Some Culinary-Medicinal Mushrooms on the Secretion of Interleukin-8 and Vascular Endothelial Growth Factor by PC3 Cancer Cells
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 7
Brenda J. Smith, Christine French, Edralin A. Lucas, Solo R. Kuvibidila, Chandra LC, Stephen L . Clarke, Stanley Lightfoot
Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
P. Pratheeshkumar, Girija Kuttan
Stimulation of Human Innate Immune Cells by Medicinal Mushroom Sclerotial Polysaccharides
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 3
Ka-Hing Wong, Peter Chi Keung Cheung, Connie K. M. Lai
Chemical Composition and Immunomodulatory Activity of Mycelia of the Hairy Bracket Mushroom, Trametes hirsuta (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 3
Rongxia Ma, Zhiyi Chen, Rongling Yang, Siyuan Wang, Chunying Yang, Xueming Liu