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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i6.40
pages 521-533

Immunomodulatory Effects of Extracellular β-Glucan Isolated from the King Oyster Mushroom Pleurotus eryngii (Agaricomycetes) and Its Sulfated Form on Signaling Molecules Involved in Innate Immunity

Yong Hyun Kim
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea; BMI Korea Co. Ltd., Uiwang-si, Gyeonggi-Do, Republic of Korea
Eui-Gil Jung
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Kook-Il Han
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Bharat Bhusan Patnaik
Trident School of Biotech Sciences, Trident Academy of Creative Technology, Chandrasekharpur, Bhubaneswar, Odisha, India
Hyun-Jung Kwon
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Hyoun-Su Lee
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea
Wan Jong Kim
BMI Korea Co. Ltd., Uiwang-si, Gyeonggi-Do, Republic of Korea
Man-Deuk Han
Department of Life Science and Biotechnology, Soonchunhyang University, Asan-si, Chungnam, Republic of Korea

RÉSUMÉ

The aim of this study was to determine, using murine RAW 264.7 macrophages, the immunomodulatory effect of extracellular β-glucan isolated from Pleurotus eryngii (PEBG) and its sulfated derivative (PEBG-S) on signaling molecules implicated in host innate immunity. β-Glucan was extracted and purified from the mycelial culture using optimal medium concentrations. It was then chemically converted to its sulfated form. Monosaccharide composition of β-glucan was characterized with p-aminobenzoic acid ethyl ester–derivatized sugars through highperformance liquid chromatography analysis. Fourier transform infrared structural analysis showed an S=O bond at 1250 cm-1 and C-S-O binding at 815 cm-1 in PEBG-S. 13C nuclear magnetic resonance analysis showed 1,3-linked α-D-mannopyranosyl and 1,3-β-D-glucopyranosyl in PEBG-S. A concentration-dependent increase of nitric oxide production was noticed in RAW 264.7 cells treated with PEBG-S or PEBG; those treated with PEBG-S showed less cytotoxicity than those treated with PEBG. Cellular levels of tumor necrosis factor-α, interleukin-1β, and interleukin-6 were increased by PEBG and PEBG-S treatment, suggesting that they have immunomodulatory activity. Real-time polymerase chain reaction array revealed that the expression levels of nuclear factor-κB and Toll-like receptor signaling genes in cells were upregulated by PEBG and PEBG-S. Moreover, the expression of the β-glucan receptor dectin-2 was significantly upregulated by PEBG and PEBG-S treatment, reflecting immune activation through the dectin-2-Syk-(CARD9/Bcl-10/MALT1) pathway. Our results suggest that PEBG-S could be used as an effective adjuvant or immune enhancer that can be sustainably produced by recycling the by-product of mycelial culture.


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