Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.211 Facteur d'impact sur 5 ans: 1.394 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v1.i1.70
pages 89-92

Flammulin Purified from the Fruit Bodies of Flammulina velutipes (Curt.:Fr.) P.Karst.

Hua Zhang
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Feng Gong
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
Yongjun Feng
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China
Changkai Zhang
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P. R. China

RÉSUMÉ

Flammulin, an antitumor substance, was purified from the aqueous extract of basidiomes of Flammulina velutipes (Curt.: Fr.) P.Karst. It was judged homogenous by SDS-polyacrylamide gel electrophoresis. The purification procedure included ammonium sulfate precipitation, ethanol fractionation, DEAE-Sephadex anion-exchange chromatography, crystallization, and recrystallization. Flammulin was crystallized with microdialysis against a polyethylene glycol sodium phosphate buffer. The crystals were rhombohedron-shaped and easy to melt when the crystallization conditions were changed. With a molecular mass of 23,700, flammulin was characterized by its lack of Met. Amino acid composition analysis showed that the numbers of acidic amino acids are more than that of basic amino acids, but the isoelectric point detected by analytical isoelectric focusing was at pH8.9.


Articles with similar content:

Regulation of Growth and Biosynthetic Activity of the Medicinal Jelly Mushroom Tremella mesenterica (Retz.: Fr.) Pure Culture
International Journal of Medicinal Mushrooms, Vol.3, 2001, issue 1
Sergey V. Reshetnikov, Ina Duckman, Katherina Tsukor
Novel Ganoderma lucidum-based beverage with antibacterial and antioxidant effects
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 3
Miomir Niksic
Isolation and Characterization of Lectins Formed by Cerrena unicolor (Higher Basidiomycetes) in Solid-State Fermentation of Sorghum and Wheat Straw
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Elene Davitashvili, Ekaterine Kapanadze, Eva Kachlishvili, Nona A. Mikiashvili, Vladimir I. Elisashvili
Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 3
Danka Matijašević, Aleksandra Sknepnek, Milena Pantić, Dunja Miletić, Miomir Niksic, Steva Lević, Viktor Nedović
Antioxidant and Gene Protective Effects of Medicinal Mushrooms Inonotus obliquus (Pers.:Fr.) Pilat and Phellinus robustus (P. Karst.) Bourd. et Galz.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Victor T. Bilay, Nina A. Bisko, Victor V. Shcherba, Nadezda Yu. Mitropolskaya