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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019031423
pages 817-823

Secondary Metabolites and Antioxidant Capacity of the Tunisian Lichen Diploschistes ocellatus (Ascomycota)

Mohamed Mendili
University of Tunis El-Manar II, Faculty of Sciences, Unit of Research of Plant Ecology, Campus Academia, 2092 Tunis, Tunisia
Marwa Bannour
University of Tunis El-Manar II, Faculty of Sciences, Unit of Research of Plant Ecology, Campus Academia, 2092 Tunis, Tunisia
Maria Eduarda M. Araújo
Faculty of Sciences, Centre of Chemistry and Biochemistry, University of Lisbon, Lisboan, Portugal
Samira Aschi-Smiti
University of Tunis El-Manar II, Faculty of Sciences, Unit of Research of Plant Ecology, Campus Academia, 2092 Tunis, Tunisia
Mark R. D. Seaward
School of Archaeological & Forensic Sciences, University of Bradford, Bradford BD7 1DP, UK
Ayda Khadhria
University of Tunis El-Manar II, Faculty of Sciences, Unit of Research of Plant Ecology, Campus Academia, 2092 Tunis, Tunisia


This study, the first to assess the total phenolic, flavonoid, tannins, and proanthocyanidin content of the Tunisian lichen Diploschistes ocellatus, determined the antioxidant capacity in scavenging 2,2 diphenyl-1-picrylhydrazyl (DPPH), as well as the ferric-reducing and iron-chelating powers. The phenolic compound content of D. ocellatus was shown to be related to antioxidant activity. The highest phenolic and flavonoid contents of extracts were obtained with acetone (286.3 μg GAE/g DW and 3.24 μg CE/g DW, respectively), while the highest tannin and proanthocyanidin contents were obtained with methanol (5.5 μg TAE/g DW and 35.12 μg CE/g DW, respectively). The highest DPPH' scavenging capacity and iron-chelating power of extracts were obtained with methanol (concentration providing 50% inhibition [IC50] = 0.029 mg/mL and IC50 = 0.425 mg/mL, respectively), while acetone extracts showed a higher reducing power (IC50 = 0.118 mg/mL).


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