Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.211 Facteur d'impact sur 5 ans: 1.394 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i2.10
pages 115-126

Analysis of the Titer and Reactivity of Antibody/ies Against Fungal Cell Wall β-Glucans in Human Sera

Ken-Ichi Ishibashi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Mayu Morita
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
Masuro Motoi
Toei Pharmaceutical Co. Ltd., 2-5-3 Iguchi, Mitaka, Tokyo 181-0011; Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
Ying Liu
Research Center for Food Safety, The University of Tokyo; Mibyou Medical Research Center, Institute of Preventive Medicine, Setagaya-ku, Tokyo, Japan
Noriko N. Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
Yoshiyuki Adachi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Naohito Ohno
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Tokyo, Japan

RÉSUMÉ

The aim of this work was to characterize an antibody response to β-glucan (BG), a major component of the fungal cell wall, at each isotype in human sera. The titer and reactivity of the anti-human BG antibody were examined using enzyme-linked immunosorbant assay plates coated with Candida soluble cell wall β-glucan as a standard antigen. The antibody was detected using anti-human immunoglobulin (Ig) G, IgM, and IgA. Its major class was IgG in all subjects. The antibody titer varied significantly. The anti-BG antibody showed greater reactivity to β-glucans derived from pathogenic fungi than monoglycosyl-branched β-glucans derived from mycelia culture medium. In addition, it was suggested that the anti-BG IgM antibody was bound relatively strongly to the β1,3-glucan backbone and the anti-BG IgG antibody to β1,6-glucan. The anti-BG antibody plays a variety of roles, according to class, in the host's response to fungi. We propose a new index of human response to β-glucan that effects the understanding of the response to β-glucan in humans.


Articles with similar content:

Anti-β-Glucan Antibody in Bovine Sera
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 4
Yoshiyuki Adachi, Noriko N. Miura, Tsunenori Iriki, Yu-ichiro Kurone, Naohito Ohno, Ken-Ichi Ishibashi, Chikaku Dogasaki, Masuro Motoi
Effect of Sasa veitchii Extract on Immunostimulating Activity of β-glucan (SCG) from Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Mia Yoshida, Naohito Ohno, Toshie H. Hida, Natsuko Akachi
Dectin-1 and GM-CSF on Immunomodulating Activities of Fungal 6-Branched 1,3-β-Glucans
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 2
Naohito Ohno, Toshie Harada
Innate Immunity Induced by Fungal β-Glucans via Dectin-1 Signaling Pathway
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 1
Ha Won Kim, Dong Hee Lee
Elimination of Virus-Specific Cytotoxic T Cells in the Liver
Critical Reviews™ in Immunology, Vol.22, 2002, issue 1
Gunther Dennert