Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v4.i3.20
9 pages

Extraction of Conformationally Stable (1®6)-Branched (1®3)-b-Glucans from Premixed Edible Mushroom Powders by Cold Alkaline Solution

Masaji Sawai
Takara Agri Co., Ltd., 2257 Noji, Kusatsu, Shiga, Japan
Yoshiyuki Adachi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Manabu Kanai
Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392;Japan
Susumu Matsui
Takara Agri Co., Ltd., 2257 Noji, Kusatsu, Shiga, Japan
Toshiro Yadomae
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan


(1®3)-b-glucan content in extracts from various edible mushrooms was measured by using a (l®-6)-branched (1®3)-b-glucan-specific enzyme immune system. Comparing (1®3)-b-glucan content in hot water extracts or cold alkaline extracts of each edible mushroom, it was shown that Lentinus edodes (Berk.) Sing. contained higher levels of (1®3)-b-glucan than any of the other mushrooms. Cold alkaline extract possessed the highest amount of (1®3)-b-glucans. Interestingly, a cold alkaline extract from premixed dried powder of the mushrooms showed a higher level of (1®3)-b-glucan following calculation as sonifilan than the theoretical total amount of each (1®3)-b-glucan extract from each constituent mushroom from which premixed dried powder was prepared. Considering the specificity of this enzyme immunoassay as previously reported, it is suggested that premixed extraction contains Conformationally stable (1®3)-b-glucans. Antitumor activity of these mushroom extracts was also tested using a murine experimental model. All of the extracts showed higher antitumor activity and the effectiveness on inhibition of tumor growth in sold form was almost equal. Activation of macrophages by co-cultivation with the various extracts was induced with the premixed extract significantly higher. Induction of interferon-y was also enhanced by administration of the premixed extract. The premix extract showed modulating effect on glucocorticoid release induced by restraint stress. These results suggest that the mixture of mushroom extracts may provide physicochemically stable (1®3)-b-glucans and physiologically active products. A complementary effect of each mushroom component on enhancing immunological function would be expected by the mixed extraction.font face=

Articles with similar content:

Structure and Antitumor Activity of 1,3-b-Glucan from Cultivated Fruit Bodies of Culinary–Medicinal Mushroom Hypsizygus marmoreus (Peck) Bigel. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 3
Shigeyuki Goto, Naohito Ohno, Masuro Motoi
Production of Polysaccharide by Culinary-Medicinal Mushroom Agaricus brasiliensis S. Wasser et al. LPB 03 (Agaricomycetideae) in Submerged Fermentation and Its Antitumor Effect
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 1
Aurora L. Pedroso, Carlos Ricardo Soccol, Leifa Fan, Renato Rau, Sandro Germano, Ashok Pandey, Andrea Thomaz Soccol
Diverse Functional Activity of CD83+ Monocyte-Derived Dendritic Cells and the Implications for Cancer Vaccines
Critical Reviews™ in Immunology, Vol.21, 2001, issue 1-3
Brian J. Czerniecki, Isabelle Bedrosian, Peter A. Cohen, Mark B. Faries, James G. Roros, Shuwen Xu
Antitumor Effects of Polysaccharides of Ganoderma lucidum (Curt.:Fr.) P. Karst. (Ling Zhi, Reishi Mushroom) (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 1
Chieh-Fu Chen, Po-Lei Lee, Kuang-Yao Chen, Sheng-Yuan Wang, Shiuh-Sheng Lee
Immunomodulating Activities of Cultivated Maitake Medicinal Mushroom Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes) on Peripheral Blood Mononuclear Cells
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 4
Sasa Simcic, Branka Wraber, Andrej Gregori, Mirjan Svagelj, Bojana Boh, Marin Berovic